|Application ||WB, FC, E|
|Calculated MW||48356 Da|
|Antigen Region||384-409 aa|
|Other Names||Interferon regulatory factor 8, IRF-8, Interferon consensus sequence-binding protein, H-ICSBP, ICSBP, IRF8, ICSBP1|
|Target/Specificity||This IRF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-409 amino acids from the C-terminal region of human IRF8.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IRF8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role as a transcriptional activator or repressor (PubMed:25122610). Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Positively regulates macroautophagy in dendritic cells (PubMed:29434592).|
|Cellular Location||Nucleus. Cytoplasm. Note=In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN- gamma induction.|
|Tissue Location||Predominantly expressed in lymphoid tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection.
McGough,J.M., Mol. Cancer Res. 6 (12), 1841-1851 (2008)
Tshuikina,M., Exp. Hematol. 36 (12), 1673-1681 (2008)
Martinez,A., Am. J. Surg. Pathol. 32 (8), 1190-1200 (2008)
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