|Application ||WB, FC, E|
|Calculated MW||114536 Da|
|Antigen Region||796-824 aa|
|Other Names||Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain, ITGA5, FNRA|
|Target/Specificity||This CD49e antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 796-824 amino acids from the C-terminal region of human CD49e.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD49e Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430).|
|Cellular Location||Membrane; Single-pass type I membrane protein. Cell junction, focal adhesion. Cell surface|
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Provided below are standard protocols that you may find useful for product applications.
CD49e belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein is the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Boehmler,A.M., J. Immunol. 182 (11), 6789-6798 (2009)
Okazaki,T., Am. J. Pathol. 174 (6), 2378-2387 (2009)
Schornberg,K.L., Proc. Natl. Acad. Sci. U.S.A. 106 (19), 8003-8008 (2009)
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