- CITATIONS: 1
|Application ||WB, FC, E|
|Other Accession||P17136, P27048, Q58DW4|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||24610 Da|
|Antigen Region||34-65 aa|
|Other Names||Small nuclear ribonucleoprotein-associated proteins B and B', snRNP-B, Sm protein B/B', Sm-B/B', SmB/B', SNRPB, COD, SNRPB1|
|Target/Specificity||This SNRPB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-65 amino acids from the N-terminal region of human SNRPB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SNRPB Antibody (N-term R49) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:18984161, PubMed:19325628, PubMed:25555158, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346). Component of both the pre- catalytic spliceosome B complex and activated spliceosome C complexes (PubMed:11991638, PubMed:28502770, PubMed:28781166, PubMed:28076346). Is also a component of the minor U12 spliceosome (PubMed:15146077). As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing (PubMed:12975319).|
|Cellular Location||Cytoplasm, cytosol. Nucleus Note=SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.|
Provided below are standard protocols that you may find useful for product applications.
SNRPB is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein.
Elkon,K.B.,et.al., J. Immunol. 145 (2), 636-643 (1990)
Chu,J.L. et.al., Gene 97 (2), 311-312 (1991)
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