Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
|Application ||DB, E|
|Other Accession||A6NCE7, NP_073729|
|Calculated MW||14688 Da|
|Other Names||Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3|
|Target/Specificity||This APG8b(MAP1LC3B) Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T93/Y99 of human APG8b(MAP1LC3B).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bi-Phospho-APG8b(MAP1LC3B)(T93/Y99) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.|
|Cellular Location||Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome Note=LC3-II binds to the autophagic membranes. Localizes also to discrete punctae along the ciliary axoneme (By similarity)|
|Tissue Location||Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver|
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Provided below are standard protocols that you may find useful for product applications.
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Rouschop, K.M., et al. J. Clin. Invest. 120(1):127-141(2010)
Kirkin, V., et al. Mol. Cell 33(4):505-516(2009)
Othman, E.Q., et al. J. Clin. Lab. Anal. 23(4):249-258(2009)
Liu, Q., et al. Ai Zheng 27(1):25-29(2008)
Komatsu, M., et al. Cell 131(6):1149-1163(2007)
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