Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
|Application ||DB, WB, E|
|Calculated MW||48337 Da|
|Other Names||G2/mitotic-specific cyclin-B1, CCNB1, CCNB|
|Target/Specificity||This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-CCNB1(S35) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for the control of the cell cycle at the G2/M (mitosis) transition.|
|Cellular Location||Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].
Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010)
van Zon, W., et al. J. Cell Biol. 190(4):587-602(2010)
Harley, M.E., et al. EMBO J. 29(14):2407-2420(2010)
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Nantajit, D., et al. PLoS ONE 5 (8), E12341 (2010) :
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