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MNT Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MNT Antibody AP50615
    Western blot analysis of lysate from A431 cell line,using MNT Antibody(AP50615). AP50615 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q99583
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 62 KDa
Antigen Region 318-351 aa
Additional Information
Other Names Max-binding protein MNT, Class D basic helix-loop-helix protein 3, bHLHd3, Myc antagonist MNT, Protein ROX, MNT, BHLHD3, ROX
Dilution WB~~ 1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name MNT
Synonyms BHLHD3, ROX
Function Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.
Cellular Location Nucleus. EMBL; X96401; CAA65265.1; -; mRNA EMBL; Y13440; CAA73851.1; -; Genomic_DNA EMBL; Y13441; CAA73851.1; JOINED; Genomic_DNA EMBL; Y13442; CAA73851.1; JOINED; Genomic_DNA EMBL; Y13443; CAA73851.1; JOINED; Genomic_DNA EMBL; Y13444; CAA73851.1; JOINED; Genomic_DNA EMBL; AK291596; BAF84285.1; -; mRNA EMBL; CH471108; EAW90541.1; -; Genomic_DNA EMBL; CH471108; EAW90542.1; -; Genomic_DNA EMBL; BC117563; AAI17564.1; -; mRNA CCDS; CCDS11018.1; - RefSeq; NP_064706.1; NM_020310.2 UniGene; Hs.626579; - UniGene; Hs.632239; - ProteinModelPortal; Q99583; - SMR; Q99583; - BioGrid; 110478; 16 ELM; Q99583; - IntAct; Q99583; 6 MINT; Q99583; - STRING; 9606.ENSP00000174618; - iPTMnet; Q99583; - PhosphoSitePlus; Q99583; - BioMuta; MNT; - DMDM; 3914034; - EPD; Q99583; - MaxQB; Q99583; - PaxDb; Q99583; - PeptideAtlas; Q99583; - PRIDE; Q99583; - ProteomicsDB; 78341; - Ensembl; ENST00000174618; ENSP00000174618; ENSG00000070444 GeneID; 4335; - KEGG; hsa:4335; - UCSC; uc002fur.4; human CTD; 4335; - DisGeNET; 4335; - EuPathDB; HostDB:ENSG00000070444.14; - GeneCards; MNT; - HGNC; HGNC:7188; MNT HPA; HPA059532; - HPA; HPA067578; - MIM; 603039; gene neXtProt; NX_Q99583; - OpenTargets; ENSG00000070444; - PharmGKB; PA30898; - eggNOG; KOG2483; Eukaryota eggNOG; ENOG4111VW9; LUCA GeneTree; ENSGT00510000048287; - HOGENOM; HOG000013115; - HOVERGEN; HBG006379; - InParanoid; Q99583; - KO; K09115; - OMA; AVIPMVP; - OrthoDB; EOG091G0PW2; - PhylomeDB; Q99583; - TreeFam; TF315654; - SIGNOR; Q99583; - ChiTaRS; MNT; human GeneWiki; MNT_(gene); - GenomeRNAi; 4335; - PRO; PR:Q99583; - Proteomes; UP000005640; Chromosome 17 Bgee; ENSG00000070444; - CleanEx; HS_MNT; - ExpressionAtlas; Q99583; baseline and differential Genevisible; Q99583; HS GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005634; C:nucleus; IDA:HPA GO; GO:0003682; F:chromatin binding; IEA:Ensembl GO; GO:0003700; F:DNA binding transcription factor activity; TAS:ProtInc GO; GO:0046983; F:protein dimerization activity; IEA:InterPro GO; GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:NTNU_SB GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0003713; F:transcription coactivator activity; TAS:ProtInc GO; GO:0003714; F:transcription corepressor activity; TAS:ProtInc GO; GO:0001227; F:transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:NTNU_SB GO; GO:0007569; P:cell aging; IEA:Ensembl GO; GO:0007275; P:multicellular organism development; TAS:ProtInc GO; GO:2001234; P:negative regulation of apoptotic signaling pathway; IEA:Ensembl GO; GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB GO; GO:0051726; P:regulation of cell cycle; IEA:Ensembl GO; GO:0006366; P:transcription by RNA polymerase II; TAS:ProtInc CDD; cd00083; HLH; 1 Gene3D; 4.10.280.10; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR036638; HLH_DNA-bd_sf Pfam; PF00010; HLH; 1 SMART; SM00353; HLH; 1 SUPFAM; SSF47459; SSF47459; 1 PROSITE; PS50888; BHLH; 1 1: Evidence at protein level; Acetylation; Complete proteome; DNA-binding; Nucleus; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation INIT_MET 1 1 Removed. CHAIN 2 582 Max-binding protein MNT /FTId=PRO_0000127281 DOMAIN 220 271 bHLH. {ECO:0000255|PROSITE- ProRule:PRU00981} REGION 271 299 Leucine-zipper MOD_RES 2 2 N-acetylserine VARIANT 109 109 A -> T (in dbSNP:rs7207965) /FTId=VAR_061258 SEQUENCE 582 AA; 62300 MW; 06AC320D79BF18A0 CRC64; MSIETLLEAA RFLEWQAQQQ QRAREEQERL RLEQEREQEQ KKANSLARLA HTLPVEEPRM EAPPLPLSPP APPPAPPPPL ATPAPLTVIP IPVVTNSPQP LPPPPPLPAA AQPLPLAPRQ PALVGAPGLS IKEPAPLPSR PQVPTPAPLL PDSKATIPPN GSPKPLQPLP TPVLTIAPHP GVQPQLAPQQ PPPPTLGTLK LAPAEEVKSS EQKKRPGGIG TREVHNKLEK NRRAHLKECF ETLKRNIPNV DDKKTSNLSV LRTALRYIQS LKRKEKEYEH EMERLAREKI ATQQRLAELK HELSQWMDVL EIDRVLRQTG QPEDDQASTS TASEGEDNID EDMEEDRAGL GPPKLSHRPQ PELLKSTLPP PSTTPAPLPP HPHPHPHSVA LPPAHLPVQQ QQPQQKTPLP APPPPPAAPA QTLVPAPAHL VATAGGGSTV IAHTATTHAS VIQTVNHVLQ GPGGKHIAHI APSAPSPAVQ LAPATPPIGH ITVHPATLNH VAHLGSQLPL YPQPVAVSHI AHTLSHQQVN GTAGLGPPAT VMAKPAVGAQ VVHHPQLVGQ TVLNPVTMVT MPSFPVSTLK LA
Citations (0)

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Background

Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.

References

Meroni G.,et al.EMBO J. 16:2892-2906(1997).
Nigro C.L.,et al.Genomics 49:275-282(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Van Damme P.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).

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$ 325.00
Cat# AP50615
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