|Reactivity||Human, Mouse, Rat|
|Calculated MW||44 KDa|
|Antigen Region||17-45 aa|
|Other Names||General transcription factor IIH subunit 2, Basic transcription factor 2 44 kDa subunit, BTF2 p44, General transcription factor IIH polypeptide 2, TFIIH basal transcription factor complex p44 subunit, GTF2H2, BTF2P44|
|Format||Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Function||Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre- initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.|
|Tissue Location||Widely expressed, with higher expression in skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C- terminus is required for a successful escape of RNAP II form the promoter.
Humbert S.,et al.EMBO J. 13:2393-2398(1994).
Zhang Q.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
van der Steege G.,et al.Eur. J. Hum. Genet. 3:87-95(1995).
Chen Q.,et al.Genomics 48:121-127(1998).
Kershnar E.,et al.J. Biol. Chem. 273:34444-34453(1998).
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