Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IFT20 Antibody (Center)   

IFT20 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - IFT20 Antibody (Center) AP5133C
    Western blot analysis of IFT20 Antibody (Center) (Cat. #AP5133c) in mouse lung tissue lysates (35ug/lane).IFT20 (arrow) was detected using the purified Pab.
  • IHC-P - IFT20 Antibody (Center) AP5133C
    IFT20 Antibody (Center) (Cat. #AP5133c) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IFT20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8IY31
Other Accession Q61025, Q58CS6
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 44-71 aa
Additional Information
Other Names Intraflagellar transport protein 20 homolog, hIFT20, IFT20
Target/Specificity This IFT20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the Central region of human IFT20.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIFT20 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IFT20
Function Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium (PubMed:16775004). Regulates the platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling (PubMed:29237719). Essential for male fertility. Plays an important role in spermatogenesis, particularly spermiogenesis, when germ cells form flagella. May play a role in the transport of flagellar proteins ODF2 and SPAG16 to build sperm flagella and in the removal of redundant sperm cytoplasm (By similarity). Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment (By similarity).
Cellular Location Golgi apparatus, cis-Golgi network {ECO:0000250|UniProtKB:Q61025}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q61025}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q61025}. Cell projection, cilium {ECO:0000250|UniProtKB:Q61025}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q61025}. Golgi apparatus {ECO:0000250|UniProtKB:Q61025}. Note=Present at the centrosomes during the cell cycle and associated with the proximal portion of the mother centriole and the lateral aspect of the daughter centriole. Associated with basal body at the base of primary cilia. Detected in the Golgi apparatus of round spermatids and late spermatocytes. Also detected in the manchette of step 10-12 spermatids. In step 14 spermatids, found in the basal body of the sperm tail. {ECO:0000250|UniProtKB:Q61025}
Tissue Location Expressed in almost all tissues. EMBL; AY224601; AAP50265.1; -; mRNA EMBL; BQ639919; -; NOT_ANNOTATED_CDS; mRNA EMBL; AC002094; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC002640; AAH02640.1; -; mRNA EMBL; BC038094; AAH38094.1; -; mRNA CCDS; CCDS32593.1; -. [Q8IY31-3] CCDS; CCDS58533.1; -. [Q8IY31-4] CCDS; CCDS58534.1; -. [Q8IY31-1] CCDS; CCDS58535.1; -. [Q8IY31-2] RefSeq; NP_001254703.1; NM_001267774.1. [Q8IY31-2] RefSeq; NP_001254704.1; NM_001267775.1. [Q8IY31-1] RefSeq; NP_001254705.1; NM_001267776.1. [Q8IY31-1] RefSeq; NP_001254706.1; NM_001267777.1. [Q8IY31-4] RefSeq; NP_001254707.1; NM_001267778.1 RefSeq; NP_777547.1; NM_174887.3. [Q8IY31-3] UniGene; Hs.705431; - UniGene; Hs.744876; - ProteinModelPortal; Q8IY31; - BioGrid; 124711; 107 CORUM; Q8IY31; - IntAct; Q8IY31; 68 MINT; Q8IY31; - STRING; 9606.ENSP00000350570; - iPTMnet; Q8IY31; - PhosphoSitePlus; Q8IY31; - BioMuta; IFT20; - DMDM; 74728279; - EPD; Q8IY31; - jPOST; Q8IY31; - MaxQB; Q8IY31; - PaxDb; Q8IY31; - PeptideAtlas; Q8IY31; - PRIDE; Q8IY31; - ProteomicsDB; 71093; - ProteomicsDB; 71094; -. [Q8IY31-2] ProteomicsDB; 71095; -. [Q8IY31-3] TopDownProteomics; Q8IY31-3; -. [Q8IY31-3] DNASU; 90410; - Ensembl; ENST00000357896; ENSP00000350570; ENSG00000109083. [Q8IY31-3] Ensembl; ENST00000395418; ENSP00000378809; ENSG00000109083. [Q8IY31-1] Ensembl; ENST00000579419; ENSP00000463322; ENSG00000109083. [Q8IY31-4] Ensembl; ENST00000585089; ENSP00000464443; ENSG00000109083. [Q8IY31-2] Ensembl; ENST00000585313; ENSP00000463138; ENSG00000109083. [Q8IY31-1] GeneID; 90410; - KEGG; hsa:90410; - UCSC; uc002hau.3; human. [Q8IY31-1] CTD; 90410; - EuPathDB; HostDB:ENSG00000109083.13; - GeneCards; IFT20; - HGNC; HGNC:30989; IFT20 HPA; HPA021376; - MIM; 614394; gene neXtProt; NX_Q8IY31; - OpenTargets; ENSG00000109083; - PharmGKB; PA142671663; - eggNOG; ENOG410KC8U; Eukaryota eggNOG; ENOG4110P7T; LUCA GeneTree; ENSGT00390000003413; - HOGENOM; HOG000230527; - HOVERGEN; HBG081781; - InParanoid; Q8IY31; - KO; K16473; - OMA; IEADQNE; - OrthoDB; 1589590at2759; - PhylomeDB; Q8IY31; - TreeFam; TF319434; - Reactome; R-HSA-5620924; Intraflagellar transport ChiTaRS; IFT20; human GeneWiki; IFT20; - GenomeRNAi; 90410; - PRO; PR:Q8IY31; - Proteomes; UP000005640; Chromosome 17 Bgee; ENSG00000109083; Expressed in 235 organ(s), highest expression level in testis ExpressionAtlas; Q8IY31; baseline and differential Genevisible; Q8IY31; HS GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell GO; GO:0005813; C:centrosome; IDA:MGI GO; GO:0097546; C:ciliary base; IEA:Ensembl GO; GO:0097542; C:ciliary tip; TAS:Reactome GO; GO:0005929; C:cilium; ISS:UniProtKB GO; GO:0005801; C:cis-Golgi network; IDA:SYSCILIA_CCNET GO; GO:0044292; C:dendrite terminus; IEA:Ensembl GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB GO; GO:0000139; C:Golgi membrane; TAS:Reactome GO; GO:0030992; C:intraciliary transport particle B; ISS:UniProtKB GO; GO:1902636; C:kinociliary basal body; IEA:Ensembl GO; GO:0005902; C:microvillus; IEA:Ensembl GO; GO:0031514; C:motile cilium; IEA:Ensembl GO; GO:0032391; C:photoreceptor connecting cilium; IEA:Ensembl GO; GO:0001750; C:photoreceptor outer segment; IEA:Ensembl GO; GO:0032420; C:stereocilium; IEA:Ensembl GO; GO:0002046; F:opsin binding; IEA:Ensembl GO; GO:0017137; F:Rab GTPase binding; IPI:ParkinsonsUK-UCL GO; GO:0055007; P:cardiac muscle cell differentiation; IEA:Ensembl GO; GO:0051642; P:centrosome localization; IBA:GO_Central GO; GO:0060271; P:cilium assembly; IMP:UniProtKB GO; GO:0090102; P:cochlea development; IEA:Ensembl GO; GO:0001736; P:establishment of planar polarity; IEA:Ensembl GO; GO:0060122; P:inner ear receptor cell stereocilium organization; IEA:Ensembl GO; GO:0042073; P:intraciliary transport; IBA:GO_Central GO; GO:0035735; P:intraciliary transport involved in cilium assembly; TAS:Reactome GO; GO:0001822; P:kidney development; IEA:Ensembl GO; GO:0061351; P:neural precursor cell proliferation; IEA:Ensembl GO; GO:0036372; P:opsin transport; IEA:Ensembl GO; GO:0035845; P:photoreceptor cell outer segment organization; IEA:Ensembl GO; GO:0061512; P:protein localization to cilium; IBA:GO_Central GO; GO:0034067; P:protein localization to Golgi apparatus; IMP:MGI GO; GO:0072659; P:protein localization to plasma membrane; IEA:Ensembl GO; GO:2000785; P:regulation of autophagosome assembly; ISS:UniProtKB GO; GO:0060828; P:regulation of canonical Wnt signaling pathway; IEA:Ensembl GO; GO:1902017; P:regulation of cilium assembly; ISS:UniProtKB GO; GO:2000583; P:regulation of platelet-derived growth factor receptor-alpha signaling pathway; ISS:UniProtKB GO; GO:0007224; P:smoothened signaling pathway; IEA:Ensembl GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB GO; GO:0008542; P:visual learning; IEA:Ensembl InterPro; IPR028172; FT20 PANTHER; PTHR31978; PTHR31978; 1 Pfam; PF14931; IFT20; 1 1: Evidence at protein level; Alternative splicing; Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Differentiation; Golgi apparatus; Reference proteome; Spermatogenesis CHAIN 1 132 Intraflagellar transport protein 20 homolog /FTId=PRO_0000249303 REGION 70 132 IFT57-binding. COILED 74 114 VAR_SEQ 1 1 M -> MTHLLLTATVTPSEQNSSREPGWETAM (in isoform 2) /FTId=VSP_020390 VAR_SEQ 72 132 AIGARNLLKSIAKQREAQQQQLQALIAEKKMQLERYRVEYE ALCKVEAEQNEFIDQFIFQK -> SLAVSPRLECTGAISAH CKLCLSDSSDSPTSPSRVGGTTGHRCSELAQIYSKAERSST AATSSPNSRKENAARKVSG (in isoform 3) /FTId=VSP_020391 VAR_SEQ 107 132 YRVEYEALCKVEAEQNEFIDQFIFQK -> NELKISLL (in isoform 4) /FTId=VSP_046026 SEQUENCE 132 AA; 15281 MW; 8C751DE18FEEB30F CRC64; MAKDILGEAG LHFDELNKLR VLDPEVTQQT IELKEECKDF VDKIGQFQKI VGGLIELVDQ LAKEAENEKM KAIGARNLLK SIAKQREAQQ QQLQALIAEK KMQLERYRVE YEALCKVEAE QNEFIDQFIF QK
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


IFT20 is part of intraflagellar transport (IFT) particles involved in ciliary process assembly. IFT20 may play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.


Follit, J.A., et al. Mol. Biol. Cell 17(9):3781-3792(2006)
Jurczyk, A., et al. J. Cell Biol. 166(5):637-643(2004)
Yin, G., et al. Mol. Biol. Rep. 30(4):255-260(2003)

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

Cat# AP5133C
Availability: Back Order
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions