|Application ||WB, IHC-P, FC, E|
|Other Accession||Q63797, Q64L94, P97371, P58238, Q4U5R3|
|Predicted||Bovine, Monkey, Mouse, Pig, Rat|
|Calculated MW||28723 Da|
|Antigen Region||220-249 aa|
|Other Names||Proteasome activator complex subunit 1, 11S regulator complex subunit alpha, REG-alpha, Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, Proteasome activator 28 subunit alpha, PA28a, PA28alpha, PSME1, IFI5111|
|Target/Specificity||This PSME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the C-terminal region of human PSME1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PSME1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.|
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Provided below are standard protocols that you may find useful for product applications.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring.
Ruckrich, T., et al. Leukemia 23(6):1098-1105(2009) Lemaire, R., et al. J. Proteome Res. 6(11):4127-4134(2007) Kobayashi, K., et al. Brain Res. 1170, 129-139 (2007)
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