|Application ||IHC-P, WB, E|
|Calculated MW||29927 Da|
|Antigen Region||197-225 aa|
|Other Names||Dehydrogenase/reductase SDR family member 2, mitochondrial, 111-, Dicarbonyl reductase HEP27, Protein D, DHRS2|
|Target/Specificity||This DHRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-225 amino acids from the C-terminal region of human DHRS2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DHRS2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2- propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21.|
|Cellular Location||Mitochondrion matrix. Nucleus. Note=A minor fraction of the protein is translocated from the mitochondria to the nucleus, after cleavage of the targeting signal|
|Tissue Location||Widely expressed, with highest levels in liver and kidney, followed by heart, spleen, skeletal muscle and placenta. In hemopoietic cells, expressed in dendritic cells, but not in monocytes, macrophages, granulocytes, nor in B and T lymphocytes.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
DHRS2 displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates. DHRS2 do not reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. This protein may inhibit cell replication.
Monge, M., et al. Carcinogenesis 30(8):1288-1297(2009)
Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009)
Shafqat, N., et al. Cell. Mol. Life Sci. 63(10):1205-1213(2006)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.