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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   ANKRD32 Antibody (N-term)   

ANKRD32 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ANKRD32 Antibody (N-term) AP5407a
    ANKRD32 Antibody (N-term)(Cat. #AP5407a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ANKRD32 antibody detected ANKRD32 protein (arrow).
  • IHC-P - ANKRD32 Antibody (N-term) AP5407a
    ANKRD32 Antibody (N-term) (Cat. #AP5407a) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKRD32 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BQI6
Other Accession NP_115666.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 121050 Da
Antigen Region 159-188 aa
Additional Information
Gene ID 84250
Other Names Ankyrin repeat domain-containing protein 32, BRCT domain-containing protein 1, ANKRD32, BRCTD1
Target/Specificity This ANKRD32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-188 amino acids from the N-terminal region of human ANKRD32.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANKRD32 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLF1 {ECO:0000303|PubMed:25931565, ECO:0000312|HGNC:HGNC:25408}
Function Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication- coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed:25931565). Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions (PubMed:25931565).
Cellular Location Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q8R3P9}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q8R3P9}. Note=Relocalizes with RAD18 to nuclear foci in response to DNA damage. Colocalizes with RAD18 in the nucleus and to centrosomes (By similarity). Associates with chromatin (PubMed:25931565). Accumulates with RAD18 and the SMC5- SMC6 complex at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin- dependent manner (PubMed:25931565). {ECO:0000250|UniProtKB:Q8R3P9, ECO:0000269|PubMed:25931565}
Research Areas
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Adams, D.J., et al. Mol. Cell. Biol. 25(2):779-788(2005)

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$ 325.00
$ 109.00
Cat# AP5407a
Availability: In Stock
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