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RDH16 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RDH16 Antibody  (N-term) AP5456a
    RDH16 Antibody (N-term) (Cat.#AP5456a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RDH16 antibody detected the RDH16 protein (arrow).
  • IHC-P - RDH16 Antibody  (N-term) AP5456a
    RDH16 Antibody (N-term (Cat. #AP5456a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH16 Antibody (N-term for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - RDH16 Antibody  (N-term) AP5456a
    RDH16 Antibody (N-term) (Cat. #AP5456a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75452
Other Accession NP_003699.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35673 Da
Antigen Region 3-31 aa
Additional Information
Gene ID 8608
Other Names Retinol dehydrogenase 16, 11--, Microsomal NAD(+)-dependent retinol dehydrogenase 4, RoDH-4, Sterol/retinol dehydrogenase, RDH16, RODH4
Target/Specificity This RDH16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-31 amino acids from the N-terminal region of human RDH16.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRDH16 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RDH16
Synonyms RODH4, SDR9C8
Function Oxidoreductase with a preference for NAD. Oxidizes all- trans-retinol and 13-cis-retinol to the corresponding aldehydes. Has higher activity towards CRBP-bound retinol than with free retinol. Oxidizes 3-alpha-hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction.
Cellular Location Microsome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein
Tissue Location Highly expressed in adult liver (at protein level). Detected in endometrium, liver and foreskin. Detected in the spineous layers of adult skin, and at lower levels in basal and granular skin layers. Detected in fetal liver and lung
Research Areas
Citations (0)

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Retinol dehydrogenase 16 (RDH16) is an oxidoreductase with a preference for NAD. It oxidizes all-trans-retinol and 13-cis-retinol to the corresponding aldehydes. RDH16 has higher activity towards CRBP-bound retinol than with free retinol. It oxidizes 3-alpha-hydroxysteroids and also oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. RDH16 can also catalyze the reverse reaction.


Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009) :
Lapshina, E.A., et al. Biochemistry 42(3):776-784(2003)
Cain, J.M., et al. Am. J. Obstet. Gynecol. 186(4):675-683(2002)

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Cat# AP5456a
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