- CITATIONS: 2
|Application ||WB, IHC-P, E|
|Calculated MW||36615 Da|
|Antigen Region||304-334 aa|
|Other Names||NAD-dependent protein deacetylase sirtuin-3, 351-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, mSIR2L3, Sirt3, Sir2l3|
|Target/Specificity||This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||M Sirt3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||NAD-dependent protein deacetylase (PubMed:23835326, PubMed:17923681, PubMed:18794531, PubMed:21172655). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:23835326, PubMed:17923681, PubMed:18794531, PubMed:21172655). Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5O (PubMed:16790548, PubMed:18794531, PubMed:21172655). Contributes to the regulation of the cellular energy metabolism (PubMed:23835326). Important for regulating tissue-specific ATP levels (PubMed:18794531, PubMed:24252090). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301).|
|Cellular Location||Isoform L: Mitochondrion matrix|
|Tissue Location||Strongly expressed in liver and kidney (PubMed:11056054). Expressed in skeletal muscles (at protein level) (PubMed:23283301, PubMed:23835326). Weakly expressed in lung (PubMed:11056054).|
Provided below are standard protocols that you may find useful for product applications.
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
Hirschey, M.D., et al. Nature 464(7285):121-125(2010)
Pillai, V.B., et al. J. Biol. Chem. 285(5):3133-3144(2010)
Kim, H.S., et al. Cancer Cell 17(1):41-52(2010)
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