|Application ||WB, IHC-P, E|
|Calculated MW||28996 Da|
|Antigen Region||77-109 aa|
|Other Names||Gamma-secretase subunit APH-1A, APH-1a, Aph-1alpha, Presenilin-stabilization factor, APH1A, PSF|
|Target/Specificity||This APH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human APH1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APH1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:12297508, PubMed:12522139, PubMed:12763021, PubMed:12679784, PubMed:25043039, PubMed:26280335). Required for normal gamma- secretase assembly (PubMed:12522139, PubMed:12471034, PubMed:12763021, PubMed:19369254). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable).|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi|
|Tissue Location||Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.|
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Provided below are standard protocols that you may find useful for product applications.
APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003).
Kimberly, W.T., et al., Proc. Natl. Acad. Sci. U.S.A. 100(11):6382-6387 (2003).
Edbauer, D., et al., Nat. Cell Biol. 5(5):486-488 (2003).
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