Metabotropic Glutamate Receptor 5 (GPRC1E) Antibody (C-term T1003)
Purified Rabbit Polyclonal Antibody (Pab)
|Application ||WB, E|
|Calculated MW||132469 Da|
|Antigen Region||1020-1050 aa|
|Other Names||Metabotropic glutamate receptor 5, mGluR5, GRM5, GPRC1E, MGLUR5|
|Target/Specificity||This Metabotropic Glutamate Receptor 5 (GPRC1E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1020-1050 amino acids from the C-terminal region of human Metabotropic Glutamate Receptor 5 (GPRC1E).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Metabotropic Glutamate Receptor 5 (GPRC1E) Antibody (C-term T1003) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 (also known as GPRC1E) and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. The activity of GRM5 is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
Pacheco, R., et al., J. Biol. Chem. 279(32):33352-33358 (2004).
Anneser, J.M., et al., Neuroreport 15(2):271-273 (2004).
Uchino, M., et al., J. Biol. Chem. 279(3):2254-2261 (2004).
Corti, C., et al., J. Biol. Chem. 278(35):33105-33119 (2003).
Aronica, E., et al., Eur. J. Neurosci. 17(10):2106-2118 (2003).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.