|Application ||WB, E|
|Calculated MW||97336 Da|
|Antigen Region||744-773 aa|
|Other Names||Cytokine receptor common subunit beta, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit, CD131, CSF2RB, IL3RB, IL5RB|
|Target/Specificity||This IL3R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 744-773 amino acids from the C-terminal region of human IL3R.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IL3RB Antibody (C-term V759) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Murphy, J.M., et al., J. Biol. Chem. 279(25):26500-26508 (2004).
McClure, B.J., et al., Blood 101(4):1308-1315 (2003).
Scibek, J.J., et al., Anal. Biochem. 307(2):258-265 (2002).
Blake, T.J., et al., J. Leukoc. Biol. 72(6):1246-1255 (2002).
Evans, C.A., et al., Blood 100(9):3164-3174 (2002).
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