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EEF1A1/ EEF1A2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EEF1A1/ EEF1A2 Antibody (N-term) AP6592a
    Western blot analysis of EEF1A1/ EEF1A2 Antibody (N-term) (Cat. #AP6592a) in Y79, T47D cell line lysates (35ug/lane). EEF1A1/ EEF1A2 (arrow) was detected using the purified Pab.
  • IHC-P - EEF1A1/ EEF1A2 Antibody (N-term) AP6592a
    Formalin-fixed and paraffin-embedded human brain with EEF1A1/ EEF1A2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - EEF1A1/ EEF1A2 Antibody (N-term) AP6592a
    Flow cytometric analysis of NCI-H292 cells using EEF1A1/ EEF1A2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - EEF1A1/ EEF1A2 Antibody (N-term) AP6592a
    With 293T cell line lysate,resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EEF1A1/ EEF1A2 ( abgent, Cat. AP6592a, 1:500, 4°C,overnight ) and horseradish peroxidase–conjugated second antibo dy(rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P68104
Other Accession P02994, Q92005, Q90835, P53013, P17508, Q5VTE0, P17507, P62632, Q71V39, P62631, Q05639, P05303, Q32PH8, P17506, P62630, P68105, P10126, P08736, P62629, P68103, P13549, A2Q0Z0
Reactivity Human
Predicted Xenopus, Bovine, Hamster, Drosophila, Horse, Mouse, Rabbit, Rat, C.Elegans, Chicken, Zebrafish, Yeast
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50141 Da
Antigen Region 46-72 aa
Additional Information
Gene ID 1915
Other Names Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7
Target/Specificity This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids of human EEF1A1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF1A1/ EEF1A2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF1A1
Synonyms EEF1A, EF1A, LENG7
Function This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
Cellular Location Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane. Note=Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340) Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580) Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934).
Tissue Location Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle
Research Areas
Citations ( 0 )


EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.


Byun,H.O., Cancer Res. 69 (11), 4638-4647 (2009)

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$ 325.00
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Cat# AP6592a
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