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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   EPB41L4B Antibody (C-term)   

EPB41L4B Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EPB41L4B Antibody (C-term) AP6851b
    Western blot analysis of EPB41L4B Antibody (C-term) (Cat. #AP6851b) in NCI-H460 cell line lysates (35ug/lane). EPB41L4B (arrow) was detected using the purified Pab.(2ug/ml)
  • IHC-P - EPB41L4B Antibody (C-term) AP6851b
    (LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with EPB41L4B Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of NCI-H292 cells using EPB41L4B Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H329
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 99712 Da
Antigen Region 650-677 aa
Additional Information
Gene ID 54566
Other Names Band 41-like protein 4B, FERM-containing protein CG1, Protein EHM2, EPB41L4B, EHM2
Target/Specificity This EPB41L4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 650-677 amino acids from the C-terminal region of human EPB41L4B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEPB41L4B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPB41L4B
Synonyms EHM2, LULU2 {ECO:0000303|PubMed:22006950
Function Up-regulates the activity of the Rho guanine nucleotide exchange factor ARHGEF18 (By similarity). Involved in the regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950). Promotes cellular adhesion, migration and motility in vitro and may play a role in wound healing (PubMed:23664528). May have a role in mediating cytoskeletal changes associated with steroid-induced cell differentiation (PubMed:14521927).
Cellular Location Cytoplasm. Cell junction, tight junction Note=Accumulates along apical cell-cell boundaries and is also detected in the cytoplasm in a punctate manner
Tissue Location Expressed at higher levels in acute wounds than chronic wounds with increased expression in healing wounds, especially at the leading wound edge (PubMed:23664528). Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in testis with lower levels in prostate and breast (PubMed:14521927)
Research Areas
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Leyne,M.,, Unpublished

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Cat# AP6851b
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