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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RASIP1 Antibody (Center)   

RASIP1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • FC - RASIP1 Antibody (Center) AP6870c
    Overlay histogram showing K562 cells stained with AP6870c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6870c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - RASIP1 Antibody (Center) AP6870c
    All lanes : Anti-RASIP1 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RASIP1 Antibody (Center) AP6870c
    Western blot analysis of RASIP1 Antibody (Center) (Cat. #AP6870c) in K562 cell line lysates (35ug/lane). RASIP1 (arrow) was detected using the purified Pab.
  • IHC-P - RASIP1 Antibody (Center) AP6870c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with RASIP1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5U651
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 651-678 aa
Additional Information
Other Names Ras-interacting protein 1, Rain, RASIP1
Target/Specificity This RASIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 651-678 amino acids from the Central region of human RASIP1.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRASIP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS- mediated transformation. Negative regulator of amino acid starvation-induced autophagy.
Cellular Location Cytoplasm, perinuclear region. Golgi apparatus, Golgi stack. Note=Associated with perinuclear vesicles. Is recruited to Golgi stacks by activated HRAS
Tissue Location Highly expressed in heart. Detected at lower levels in placenta and pancreas. EMBL; AY378097; AAR24580.1; -; mRNA EMBL; BC028614; AAH28614.1; -; mRNA CCDS; CCDS12731.1; - RefSeq; NP_060275.2; NM_017805.2 UniGene; Hs.233955; - PDB; 5KHO; X-ray; 2.78 A; A/B=134-285 PDB; 5KHQ; X-ray; 2.80 A; A/B=134-285 PDBsum; 5KHO; - PDBsum; 5KHQ; - ProteinModelPortal; Q5U651; - SMR; Q5U651; - BioGrid; 120263; 17 DIP; DIP-29457N; - IntAct; Q5U651; 17 STRING; 9606.ENSP00000222145; - iPTMnet; Q5U651; - PhosphoSitePlus; Q5U651; - BioMuta; RASIP1; - DMDM; 74736209; - EPD; Q5U651; - PaxDb; Q5U651; - PeptideAtlas; Q5U651; - PRIDE; Q5U651; - ProteomicsDB; 65240; - Ensembl; ENST00000222145; ENSP00000222145; ENSG00000105538 GeneID; 54922; - KEGG; hsa:54922; - UCSC; uc002pki.4; human CTD; 54922; - DisGeNET; 54922; - EuPathDB; HostDB:ENSG00000105538.8; - GeneCards; RASIP1; - HGNC; HGNC:24716; RASIP1 HPA; HPA077251; - MIM; 609623; gene neXtProt; NX_Q5U651; - OpenTargets; ENSG00000105538; - PharmGKB; PA134875519; - eggNOG; KOG0160; Eukaryota eggNOG; COG5022; LUCA GeneTree; ENSGT00530000063674; - HOGENOM; HOG000154071; - HOVERGEN; HBG079536; - InParanoid; Q5U651; - OMA; QELPANY; - OrthoDB; EOG091G01MA; - PhylomeDB; Q5U651; - TreeFam; TF315987; - SignaLink; Q5U651; - ChiTaRS; RASIP1; human GenomeRNAi; 54922; - PRO; PR:Q5U651; - Proteomes; UP000005640; Chromosome 19 Bgee; ENSG00000105538; - CleanEx; HS_RASIP1; - ExpressionAtlas; Q5U651; baseline and differential Genevisible; Q5U651; HS GO; GO:0005795; C:Golgi stack; IEA:UniProtKB-SubCell GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell GO; GO:0001525; P:angiogenesis; ISS:UniProtKB GO; GO:0048754; P:branching morphogenesis of an epithelial tube; ISS:UniProtKB GO; GO:0010507; P:negative regulation of autophagy; IMP:BHF-UCL GO; GO:0043087; P:regulation of GTPase activity; ISS:UniProtKB GO; GO:0007165; P:signal transduction; IEA:InterPro GO; GO:0001570; P:vasculogenesis; ISS:UniProtKB CDD; cd15472; Myo5p-like_CBD_Rasip1; 1 InterPro; IPR037983; CBD_Rasip1/Radil InterPro; IPR002710; Dilute_dom InterPro; IPR000159; RA_dom InterPro; IPR008984; SMAD_FHA_dom_sf InterPro; IPR029071; Ubiquitin-like_domsf Pfam; PF01843; DIL; 1 Pfam; PF00788; RA; 1 SMART; SM01132; DIL; 1 SMART; SM00314; RA; 1 SUPFAM; SSF49879; SSF49879; 1 SUPFAM; SSF54236; SSF54236; 1 PROSITE; PS51126; DILUTE; 1 PROSITE; PS50200; RA; 1 1: Evidence at protein level; 3D-structure; Angiogenesis; Complete proteome; Cytoplasm; Golgi apparatus; Methylation; Phosphoprotein; Polymorphism; Reference proteome CHAIN 1 963 Ras-interacting protein 1 /FTId=PRO_0000097163 DOMAIN 144 259 Ras-associating. {ECO:0000255|PROSITE- ProRule:PRU00166} DOMAIN 600 897 Dilute. {ECO:0000255|PROSITE- ProRule:PRU00503} COMPBIAS 59 65 Poly-Pro MOD_RES 94 94 Omega-N-methylarginine {ECO:0000250|UniProtKB:Q3U0S6} MOD_RES 188 188 Phosphoserine MOD_RES 280 280 Phosphoserine {ECO:0000250|UniProtKB:Q3U0S6} MOD_RES 292 292 Phosphoserine {ECO:0000250|UniProtKB:Q3U0S6} MOD_RES 326 326 Phosphoserine {ECO:0000250|UniProtKB:Q3U0S6} MOD_RES 328 328 Phosphoserine {ECO:0000250|UniProtKB:Q3U0S6} MOD_RES 331 331 Phosphoserine {ECO:0000250|UniProtKB:Q3U0S6} MOD_RES 419 419 Phosphoserine VARIANT 601 601 R -> C (in dbSNP:rs2287922) /FTId=VAR_051302 CONFLICT 500 500 P -> R (in Ref. 1; AAR24580) CONFLICT 511 517 PPGPGWA -> RQPWLG (in Ref. 1; AAR24580) CONFLICT 736 737 EL -> DW (in Ref. 1; AAR24580) STRAND 146 152 {ECO:0000244|PDB:5KHO} STRAND 159 166 {ECO:0000244|PDB:5KHO} HELIX 172 180 {ECO:0000244|PDB:5KHO} TURN 181 184 {ECO:0000244|PDB:5KHO} HELIX 199 201 {ECO:0000244|PDB:5KHO} STRAND 202 209 {ECO:0000244|PDB:5KHO} STRAND 224 228 {ECO:0000244|PDB:5KHO} HELIX 235 241 {ECO:0000244|PDB:5KHO} STRAND 242 244 {ECO:0000244|PDB:5KHO} STRAND 249 256 {ECO:0000244|PDB:5KHO} HELIX 257 264 {ECO:0000244|PDB:5KHO} SEQUENCE 963 AA; 103457 MW; 470021F91B59EC52 CRC64; MLSGERKEGG SPRFGKLHLP VGLWINSPRK QLAKLGRRWP SAASVKSSSS DTGSRSSEPL PPPPPHVELR RVGAVKAAGG ASGSRAKRIS QLFRGSGTGT TGSSGAGGPG TPGGAQRWAS EKKLPELAAG VAPEPPLATR ATAPPGVLKI FGAGLASGAN YKSVLATARS TARELVAEAL ERYGLAGSPG GGPGESSCVD AFALCDALGR PAAAGVGSGE WRAEHLRVLG DSERPLLVQE LWRARPGWAR RFELRGREEA RRLEQEAFGA ADSEGTGAPS WRPQKNRSRA ASGGAALASP GPGTGSGAPA GSGGKERSEN LSLRRSVSEL SLQGRRRRQQ ERRQQALSMA PGAADAQIGT ADPGDFDQLT QCLIQAPSNR PYFLLLQGYQ DAQDFVVYVM TREQHVFGRG GNSSGRGGSP APYVDTFLNA PDILPRHCTV RAGPEHPAMV RPSRGAPVTH NGCLLLREAE LHPGDLLGLG EHFLFMYKDP RTGGSGPARP PWLPARPGAT PPGPGWAFSC RLCGRGLQER GEALAAYLDG REPVLRFRPR EEEALLGEIV RAAAAGSGDL PPLGPATLLA LCVQHSAREL ELGHLPRLLG RLARLIKEAV WEKIKEIGDR QPENHPEGVP EVPLTPEAVS VELRPLMLWM ANTTELLSFV QEKVLEMEKE ADQEDPQLCN DLELCDEAMA LLDEVIMCTF QQSVYYLTKT LYSTLPALLD SNPFTAGAEL PGPGAELGAM PPGLRPTLGV FQAALELTSQ CELHPDLVSQ TFGYLFFFSN ASLLNSLMER GQGRPFYQWS RAVQIRTNLD LVLDWLQGAG LGDIATEFFR KLSMAVNLLC VPRTSLLKAS WSSLRTDHPT LTPAQLHHLL SHYQLGPGRG PPAAWDPPPA EREAVDTGDI FESFSSHPPL ILPLGSSRLR LTGPVTDDAL HRELRRLRRL LWDLEQQELP ANYRHGPPVA TSP
Research Areas
Citations ( 0 )


RASIP1 may act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.


Mitin,N.Y.,, J. Biol. Chem. 279 (21), 22353-22361 (2004)

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Cat# AP6870c
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