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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PKC nu Antibody   

PKC nu Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PKC nu Antibody AP7025A
    Western blot analysis of anti-PKCnu Pab (Cat. #AP7025a) in lysate of HL60 cells stimulated with PMA (lane A) and mouse brain tissue lysate (lane B). PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • WB - PKC nu Antibody AP7025A
    Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (AP7025a) and C-term (AP7025b) antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using AP7025a and AP7025b antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.
  • IHC-P - PKC nu Antibody AP7025A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O94806
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 100471 Da
Antigen Region 352-384 aa
Additional Information
Gene ID 23683
Other Names Serine/threonine-protein kinase D3, Protein kinase C nu type, Protein kinase EPK2, nPKC-nu, PRKD3, EPK2, PRKCN
Target/Specificity This PKC-nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-384 amino acids from human PKC-nu.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPKC nu Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRKD3
Synonyms EPK2, PRKCN
Function Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity).
Cellular Location Cytoplasm. Membrane. Note=Translocation to the cell membrane is required for kinase activation
Tissue Location Ubiquitous.
Research Areas
Citations ( 0 )


Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.


Yeaman, C., et al., Nat. Cell Biol. 6(2):106-112 (2004).
Rey, O., et al., J. Biol. Chem. 278(26):23773-23785 (2003).
Matthews, S.A., et al., J. Biol. Chem. 278(11):9086-9091 (2003).
Bennasser, Y., et al., Virology 303(1):174-180 (2002).
Bennasser, Y., et al., FASEB J. 16(6):546-554 (2002).

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$ 325.00
$ 109.00
Cat# AP7025A
Availability: In Stock
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