|Application ||IHC-P, WB, E|
|Calculated MW||36085 Da|
|Antigen Region||286-317 aa|
|Other Names||Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinase, PBK, TOPK|
|Target/Specificity||This PBK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-317 amino acids from the C-terminal region of human PBK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PBK Antibody (C-term C300) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.|
|Tissue Location||Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.|
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Provided below are standard protocols that you may find useful for product applications.
PBK is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
Nandi, A., et al., Blood Cells Mol. Dis. 32(1):240-245 (2004).
Simons-Evelyn, M., et al., Blood Cells Mol. Dis. 27(5):825-829 (2001).
Zhao, S., et al., Int. J. Biochem. Cell Biol. 33(6):631-636 (2001).
Abe, Y., et al., J. Biol. Chem. 275(28):21525-21531 (2000).
Gaudet, S., et al., Proc. Natl. Acad. Sci. U.S.A. 97(10):5167-5172 (2000).
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