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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ZPK Antibody (C-term)   

ZPK Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ZPK Antibody (C-term) AP7214a
    All lanes : Anti-ZPK Antibody (C-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: Mouse brain lysate Lane 4: Mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - ZPK Antibody (C-term) AP7214a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q12852
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 93219 Da
Antigen Region 828-859 aa
Additional Information
Gene ID 7786
Other Names Mitogen-activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, DLK, Leucine-zipper protein kinase, ZPK, MAPK-upstream kinase, MUK, Mixed lineage kinase, MAP3K12, ZPK
Target/Specificity This ZPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 828-859 amino acids from the C-terminal region of human ZPK.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZPK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP3K12
Synonyms ZPK
Function Part of a non-canonical MAPK signaling pathway (PubMed:28111074). Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1 (PubMed:28111074). May be an activator of the JNK/SAPK pathway.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q60700}. Cell membrane {ECO:0000250|UniProtKB:Q60700}. Note=Behaves essentially as an integral membrane protein {ECO:0000250|UniProtKB:Q60700}
Tissue Location Highly expressed in brain and kidney.
Research Areas
Citations ( 0 )


ZPK is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.


Itoh, A., et al., Biochem. Biophys. Res. Commun. 313(3):612-618 (2004).
Fukuyama, K., et al., J. Biol. Chem. 275(28):21247-21254 (2000).
Reddy, U.R., et al., Oncogene 18(31):4474-4484 (1999).
Mata, M., et al., J. Biol. Chem. 271(28):16888-16896 (1996).
Hirai, S., et al., Oncogene 12(3):641-650 (1996).

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$ 325.00
$ 109.00
Cat# AP7214a
Availability: In Stock
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