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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   KLF5 Antibody (C-term)   

KLF5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - KLF5 Antibody (C-term) AP7342b
    KLF5 Antibody (C-term) (Cat. #AP7342b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KLF5 antibody detected the KLF5 protein (arrow).
  • IF - KLF5 Antibody (C-term) AP7342b
    Fluorescent confocal image of U251 cell stained with KLF5 Antibody (C-term)(Cat#AP7342b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF5 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.
  • IHC-P - KLF5 Antibody (C-term) AP7342b
    KLF5 Antibody (C-term) (Cat.#AP7342b) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLF5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - KLF5 Antibody (C-term) AP7342b
    KLF5 Antibody (C-term) (Cat. #AP7342b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13887
Other Accession Q9Z0Z7
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50792 Da
Antigen Region 341-370 aa
Additional Information
Gene ID 688
Other Names Krueppel-like factor 5, Basic transcription element-binding protein 2, BTE-binding protein 2, Colon krueppel-like factor, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Transcription factor BTEB2, KLF5, BTEB2, CKLF, IKLF
Target/Specificity This KLF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human KLF5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLF5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLF5
Synonyms BTEB2, CKLF, IKLF
Function Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Cellular Location Nucleus.
Tissue Location Expressed only in testis and placenta.
Research Areas
Citations ( 0 )


KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, the protein is thought to be a transcription factor.


Guo,P., Dong,X.Y. J. Biol. Chem. 284 (10), 6071-6078 (2009)
Lee,M.Y., Moon,J.S. Biochem. J. 417 (1), 313-322 (2009)
Du,J.X., Bialkowska,A.B. J. Biol. Chem. 283 (46), 31991-32002 (2008)
Miyamoto,S., Suzuki,T. Mol. Cell. Biol. 23 (23), 8528-8541 (2003)
Shi,H., Zhang,Z. Nucleic Acids Res. 27 (24), 4807-4815 (1999)

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$ 325.00
$ 109.00
Cat# AP7342b
Availability: In Stock
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