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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   SMYD3 Antibody (Center)   

SMYD3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SMYD3 Antibody (Center) AP7344c
    Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody (Center)(Cat. #AP7344c). AP7344c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - SMYD3 Antibody (Center) AP7344c
    Western blot analysis of SMYD3 antibody (Center) (Cat.#AP7344c) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab.
  • FC - SMYD3 Antibody (Center) AP7344c
    SMYD3 Antibody (Center) (Cat. #AP7344c) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H7B4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49097 Da
Antigen Region 209-236 aa
Additional Information
Gene ID 64754
Other Names Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, SMYD3, ZMYND1, ZNFN3A1
Target/Specificity This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-236 amino acids from the Central region of human SMYD3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMYD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMYD3
Synonyms ZMYND1, ZNFN3A1
Function Histone methyltransferase. Specifically methylates 'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068). Also methylates 'Lys-5' of histone H4 (PubMed:22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609). Binds DNA containing 5'- CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609).
Cellular Location Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
Tissue Location Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
Research Areas
Citations (0)

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SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.


Zou,J.N., Wang,S.Z. Cancer Lett. 280 (1), 78-85 (2009)
Wang,S.Z., Luo,X.G. BMB Rep 41 (4), 294-299 (2008)
Wang,H., Liu,Y. Cancer Sci. 99 (4), 787-791 (2008)
Barlesi,F., Giaccone,G. Int. J. Cancer 122 (6), 1441-1442 (2008)
Hamamoto,R., Silva,F.P. Cancer Sci. 97 (2), 113-118 (2006)

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$ 325.00
$ 109.00
Cat# AP7344c
Availability: In Stock
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