|Application ||IHC-P, WB, E|
|Calculated MW||137457 Da|
|Antigen Region||1-30 aa|
|Other Names||5-oxoprolinase, 5-oxo-L-prolinase, 5-OPase, Pyroglutamase, OPLAH|
|Target/Specificity||This OPLAH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human OPLAH.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||OPLAH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the cleavage of 5-oxo-L-proline to form L- glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.|
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Provided below are standard protocols that you may find useful for product applications.
OPLAH catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.
Watanabe T., Abe K.Biol. Pharm. Bull. 27:288-294(2004)
Bechtel S., Rosenfelder H.BMC Genomics 8:399-399(2007)
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