|Application ||FC, IHC-P, WB, E|
|Calculated MW||228544 Da|
|Antigen Region||1816-1845 aa|
|Other Names||Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1|
|Target/Specificity||This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1816-1845 amino acids from the C-terminal region of human PCM-1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PCM-1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for centrosome assembly and function (PubMed:12403812, PubMed:15659651, PubMed:16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed:12403812, PubMed:15659651). Required to anchor microtubules to the centrosome (PubMed:12403812, PubMed:15659651). Involved in the biogenesis of cilia (PubMed:20551181, PubMed:24121310).|
|Cellular Location||Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, cilium basal body. Note=Recruitment to the centrosome requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase Displaced from centriolar satellites and centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAP kinase signaling|
|Tissue Location||Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.|
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Provided below are standard protocols that you may find useful for product applications.
PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.
Balczon R., Bao L.J. Cell Biol. 124:783-793(1994)
Corvi R., Berger N.Oncogene 19:4236-4242(2000)
Dammermann A., Merdes A.J. Cell Biol. 159:255-266(2002)
Reiter A., Walz C.Cancer Res. 65:2662-2667(2005)
Murati A., Gelsi-Boyer V.Leukemia 19:1692-1696(2005)
Hames R.S., Crookes R.E.Mol. Biol. Cell 16:1711-1724(2005)
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