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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NRAS Antibody (C-term)   

NRAS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NRAS Antibody (C-term) AP7745b
    Anti-NRAS Antibody (C-term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P01111
Other Accession Q2MJK3
Reactivity Human
Predicted Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21229 Da
Antigen Region 147-179 aa
Additional Information
Gene ID 4893
Other Names GTPase NRas, Transforming protein N-Ras, NRAS, HRAS1
Target/Specificity This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-179 amino acids from the C-terminal region of human NRAS.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNRAS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NRAS
Synonyms HRAS1
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid- anchor Note=Shuttles between the plasma membrane and the Golgi apparatus
Research Areas
Citations (0)

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Background

NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).

References

Smalley,K.S., Cancer Res. 68 (14), 5743-5752 (2008)
Banerji,U., Mol. Cancer Ther. 7 (4), 737-739 (2008)

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$ 325.00
$ 109.00
Cat# AP7745b
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