|Application ||WB, IHC-P, E|
|Other Accession||Q6SA80, O77683, P61588|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||27368 Da|
|Antigen Region||133-165 aa|
|Other Names||Rho-related GTP-binding protein RhoE, Protein MemB, Rho family GTPase 3, Rho-related GTP-binding protein Rho8, Rnd3, RND3, ARHE, RHO8, RHOE|
|Target/Specificity||This ARHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the Central region of human ARHE.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ARHE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ARHE, RHO8, RHOE|
|Function||Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.|
|Cellular Location||Golgi apparatus membrane; Peripheral membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).
Pinner,S., Nat. Cell Biol. 10 (2), 127-137 (2008)
Poch,E., Exp. Cell Res. 313 (4), 719-731 (2007)
Ongusaha,P.P., Curr. Biol. 16 (24), 2466-2472 (2006)
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