|Application ||WB, IHC-P, E|
|Calculated MW||101116 Da|
|Antigen Region||872-899 aa|
|Other Names||Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase, MetRS, MARS|
|Target/Specificity||This MARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 872-899 amino acids from the C-terminal region of human MARS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.|
|Cellular Location||Cytoplasm, cytosol|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Methionyl-tRNA synthetase belongs to the class I family of tRNA synthetases. It catalyzes the chemical reaction: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Kaminska,M., Biochemistry 40 (47), 14309-14316 (2001)
Kang,J., J. Biol. Chem. 275 (41), 31682-31688 (2000)
Ko,Y.G., J. Cell Biol. 149 (3), 567-574 (2000)
Quevillon,S., J. Mol. Biol. 285 (1), 183-195 (1999)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.