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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   cGKII Antibody (C-term)   

cGKII Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - cGKII Antibody (C-term) AP8001a
    Western blot analysis of cGKII (arrow) using cGKII Antibody (C-term) (Cat.#AP8001a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKG2 gene (Lane 2).
  • IHC-P - cGKII Antibody (C-term) AP8001a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - cGKII Antibody (C-term) AP8001a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with cGKII antibody (C-term) (Cat.#AP8001a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13237
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 87432 Da
Antigen Region 714-744 aa
Additional Information
Gene ID 5593
Other Names cGMP-dependent protein kinase 2, cGK 2, cGK2, cGMP-dependent protein kinase II, cGKII, PRKG2, PRKGR2
Target/Specificity This cGKII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714-744 amino acids from the C-terminal region of human cGKII.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionscGKII Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRKG2
Synonyms PRKGR2
Function Crucial regulator of intestinal secretion and bone growth (By similarity). Phosphorylates and activates CFTR on the plasma membrane. Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum (By similarity). Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863 (By similarity). Acts as regulator of gene expression and activator of the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2 in mechanically stimulated osteoblasts. Under fluid shear stress, mediates ERK activation and subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2 and FOSB that play a key role in the osteoblast anabolic response to mechanical stimulation (By similarity).
Cellular Location Apical cell membrane; Lipid-anchor
Tissue Location Highly concentrated in brain, lung and intestinal mucosa
Research Areas
Citations ( 0 )


cGKII is thought to play a key role in a diverse set of physiological pathway. cGKII may mediate intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Edentification of the pathway that mediates intestinal fluid secretion by E. coli STa has potential medical implications because STa causes traveler's diarrhea and about 50% of infant mortality in developing countries. Transfection experiments in human cells disclose that cGKII phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. Impaired differentiation of cultured KMI chondrocytes can be restored by silencing Sox9 by RNA interference. cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.


Vaandrager, A.B., et al., J. Biol. Chem. 278(31):28651-28658 (2003).
Gambaryan, S., et al., Biochem. Biophys. Res. Commun. 293(5):1438-1444 (2002).
Orstavik, S., et al., Biochem. Biophys. Res. Commun. 220(3):759-765 (1996).
Fujii, M., et al., FEBS Lett. 375(3):263-267 (1995).

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$ 325.00
$ 109.00
Cat# AP8001a
Availability: In Stock
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