|Application ||IHC-P, WB, E|
|Other Accession||P97874, Q6P490|
|Calculated MW||143191 Da|
|Antigen Region||1140-1170 aa|
|Other Names||Cyclin-G-associated kinase, GAK|
|Target/Specificity||This GAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1140-1170 amino acids from the C-terminal region of human GAK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GAK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.|
|Cellular Location||Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions|
|Tissue Location||Ubiquitous. Highest in testis.|
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Provided below are standard protocols that you may find useful for product applications.
GAK, a member of the Ser/Thr protein kinase family, associates with cyclin G and CDK5. It appears to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. GAK localizes to the perinuclear area and to the trans-Golgi network. It is also observed on the plasma membrane, probably at focals adhesions. Expression is ubiquitous, wiht highest levels in testis. The protein contains 1 J domain and 1 tensin domain.
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Greener, T., et al., J. Biol. Chem. 275(2):1365-1370 (2000).
Kimura, S.H., et al., Genomics 44(2):179-187 (1997).
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