- CITATIONS: 1
|Application ||IHC-P, WB, E|
|Calculated MW||86700 Da|
|Antigen Region||720-750 aa|
|Other Names||Serine/threonine-protein kinase tousled-like 1, PKU-beta, Tousled-like kinase 1, TLK1, KIAA0137|
|Target/Specificity||This TLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720-750 amino acids from the C-terminal region of human TLK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TLK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S- phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.|
|Tissue Location||Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.|
Provided below are standard protocols that you may find useful for product applications.
The Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM]
Krause, D.R., et al., Oncogene 22(38):5927-5937 (2003).
Cabaniols, J.P., et al., Mol. Biol. Cell 10(12):4033-4041 (1999).
Sillje, H.H., et al., EMBO J. 18(20):5691-5702 (1999).
Yamakawa, A., et al., Gene 202 (1-2), 193-201 (1997).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.