- CITATIONS: 1
|Application ||WB, FC, E|
|Calculated MW||42319 Da|
|Other Names||Dual specificity protein phosphatase 6, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, MKP-3, Dusp6, Mkp3|
|Target/Specificity||This Rat MKP3 antibody is generated from rabbits immunized with a 6xHIS-tagged recombinant protein encoding aa 1~381 of rat MKP3.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||R MKP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inactivates MAP kinases. Has a specificity for the ERK family (PubMed:8626780). Implicated in muscle and neuronal differentiation (PubMed:8631996). Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (By similarity).|
|Tissue Location||Expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis|
Provided below are standard protocols that you may find useful for product applications.
Dual specificity protein tyrosine phosphatases (dsPTPs) are a subfamily of protein tyrosine phosphatases implicated in the regulation of mitogen-activated protein kinase (MAPK). MKP-1 (also known as CL100, 3CH134, Erp, and hVH-1) exemplifies a class of dual-specificity phosphatase able to reverse the activation of MAP kinases by dephosphorylating critical tyrosine and threonine residues. MKP-3 is 36% identical to MKP-1 and it blocks both the phosphorylation and enzymatic activation of ERK2 by mitogens. MKP-3 mRNA is expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis
Muda,M., et al. J. Biol. Chem. 271 (8), 4319-4326 (1996)
Mourey,R.J., et al. J. Biol. Chem. 271 (7), 3795-3802 (1996)
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