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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CTDSP1-V250 Antibody (C-term)   

CTDSP1-V250 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CTDSP1-V250 Antibody (C-term) AP8461b
    The anti-CTDSP1-V250 (Cat. #AP8461b) is used in Western blot to detect CTDSP1-V250 in CEM tissue lysate.
  • WB - CTDSP1-V250 Antibody (C-term) AP8461b
    Western blot analysis of CTDSP1 (arrow) using CTDSP1-V250 Antibody (C-term) (Cat.#AP8461b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTDSP1 gene (Lane 2) (Origene Technologies).
  • IHC-P - CTDSP1-V250 Antibody (C-term) AP8461b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9GZU7
Other Accession P58466
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29203 Da
Antigen Region 235-261 aa
Additional Information
Gene ID 58190
Other Names Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, Nuclear LIM interactor-interacting factor 3, NLI-IF, NLI-interacting factor 3, Small C-terminal domain phosphatase 1, SCP1, Small CTD phosphatase 1, CTDSP1, NIF3, NLIIF, SCP1
Target/Specificity This CTDSP1-V250 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-261 amino acids from the C-terminal region of human CTDSP1-V250.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTDSP1-V250 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms NIF3, NLIIF, SCP1
Function Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.
Cellular Location Nucleus. Note=Colocalizes with RNA polymerase II
Tissue Location Expression is restricted to non-neuronal tissues. Highest expression in skeletal muscle, spleen, lung and placenta.
Research Areas
Citations (0)

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CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF; 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.


Yeo,M., Lee,S.K., Lee,B., Ruiz,E.C., Pfaff,S.L. and Gill,G.N. Science 307 (5709): 596-600 (2005).
Fernandes,A.O., Campagnoni,C.W., Kampf,K., Feng,J.M., Handley,V.W. J. Neurosci. Res. 75 (4): 461-471 (2004).
Yeo,M., Lin,P.S., Dahmus,M.E. and Gill,G.N. J. Biol. Chem. 278 (28): 26078-26085 (2003).

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$ 325.00
$ 109.00
Cat# AP8461b
Availability: In Stock
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