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MLX Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MLX Antibody (Center) AP8638c
    Western blot analysis of MLX Antibody (Center) (Cat. #AP8638c) in MDA-MB231 cell line lysates (35ug/lane). MLX (arrow) was detected using the purified Pab.
  • IHC-P - MLX Antibody (Center) AP8638c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with MLX Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - MLX Antibody (Center) AP8638c
    Flow cytometric analysis of NCI-H292 cells using MLX Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - MLX Antibody (Center) AP8638c
    Western blot analysis of MLX (arrow) using rabbit polyclonal MLX Antibody (Center) (Cat. #AP8638c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLX gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UH92
Other Accession O08609
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 125-151 aa
Additional Information
Other Names Max-like protein X, Class D basic helix-loop-helix protein 13, bHLHd13, Max-like bHLHZip protein, Protein BigMax, Transcription factor-like protein 4, MLX, BHLHD13, TCFL4
Target/Specificity This MLX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-151 amino acids from the Central region of human MLX.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMLX Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MLX
Synonyms BHLHD13, TCFL4
Function Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
Cellular Location Isoform Alpha: Cytoplasm. Note=Found predominantly in the cytoplasm (PubMed:10918583). Isoform Gamma: Nucleus. Note=Found predominantly in the nucleus (PubMed:10918583).
Tissue Location Expressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung EMBL; AF203978; AAF14638.1; -; mRNA EMBL; AF213666; AAG40145.1; -; mRNA EMBL; AF213667; AAG40146.1; -; mRNA EMBL; AF213668; AAG40147.1; -; mRNA EMBL; AK000150; BAA90977.1; -; mRNA EMBL; AK290258; BAF82947.1; -; mRNA EMBL; AK314378; BAG37005.1; -; mRNA EMBL; AK315432; BAG37820.1; -; mRNA EMBL; BT009812; AAP88814.1; -; mRNA EMBL; BC010689; AAH10689.1; -; mRNA CCDS; CCDS11430.1; -. [Q9UH92-1] CCDS; CCDS42341.1; -. [Q9UH92-2] CCDS; CCDS45687.1; -. [Q9UH92-3] PIR; JC5333; JC5333 RefSeq; NP_733752.1; NM_170607.2. [Q9UH92-1] RefSeq; NP_937847.1; NM_198204.1. [Q9UH92-3] RefSeq; NP_937848.1; NM_198205.1. [Q9UH92-2] UniGene; Hs.383019; - UniGene; Hs.714749; - ProteinModelPortal; Q9UH92; - SMR; Q9UH92; - BioGrid; 112805; 27 IntAct; Q9UH92; 31 MINT; Q9UH92; - STRING; 9606.ENSP00000246912; - BindingDB; Q9UH92; - ChEMBL; CHEMBL2062357; - iPTMnet; Q9UH92; - PhosphoSitePlus; Q9UH92; - BioMuta; MLX; - DMDM; 20138856; - EPD; Q9UH92; - PaxDb; Q9UH92; - PeptideAtlas; Q9UH92; - PRIDE; Q9UH92; - ProteomicsDB; 84286; - ProteomicsDB; 84287; -. [Q9UH92-2] ProteomicsDB; 84288; -. [Q9UH92-3] DNASU; 6945; - Ensembl; ENST00000246912; ENSP00000246912; ENSG00000108788. [Q9UH92-1] Ensembl; ENST00000346833; ENSP00000320913; ENSG00000108788. [Q9UH92-2] Ensembl; ENST00000435881; ENSP00000416627; ENSG00000108788. [Q9UH92-3] GeneID; 6945; - KEGG; hsa:6945; - UCSC; uc002iaf.4; human. [Q9UH92-1] CTD; 6945; - DisGeNET; 6945; - EuPathDB; HostDB:ENSG00000108788.11; - GeneCards; MLX; - HGNC; HGNC:11645; MLX HPA; CAB025329; - HPA; HPA052766; - HPA; HPA064438; - MalaCards; MLX; - MIM; 602976; gene neXtProt; NX_Q9UH92; - OpenTargets; ENSG00000108788; - Orphanet; 3287; Takayasu arteritis PharmGKB; PA36397; - eggNOG; KOG1319; Eukaryota eggNOG; ENOG410ZWIP; LUCA GeneTree; ENSGT00530000063219; - HOVERGEN; HBG019061; - InParanoid; Q9UH92; - KO; K09113; - OMA; IMEEMFE; - OrthoDB; EOG091G0RUK; - PhylomeDB; Q9UH92; - TreeFam; TF318841; - Reactome; R-HSA-163765; ChREBP activates metabolic gene expression ChiTaRS; MLX; human GeneWiki; MLX_(gene); - GenomeRNAi; 6945; - PRO; PR:Q9UH92; - Proteomes; UP000005640; Chromosome 17 Bgee; ENSG00000108788; - CleanEx; HS_MLX; - ExpressionAtlas; Q9UH92; baseline and differential Genevisible; Q9UH92; HS GO; GO:0005737; C:cytoplasm; IDA:UniProtKB GO; GO:0005829; C:cytosol; IDA:HPA GO; GO:0031965; C:nuclear membrane; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005634; C:nucleus; IDA:UniProtKB GO; GO:0003677; F:DNA binding; IDA:BHF-UCL GO; GO:0003700; F:DNA binding transcription factor activity; NAS:UniProtKB GO; GO:0046982; F:protein heterodimerization activity; IPI:BHF-UCL GO; GO:0042803; F:protein homodimerization activity; IPI:BHF-UCL GO; GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:NTNU_SB GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0008134; F:transcription factor binding; IPI:BHF-UCL GO; GO:0001227; F:transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:NTNU_SB GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:NTNU_SB GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:BHF-UCL GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW CDD; cd00083; HLH; 1 Gene3D;; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR036638; HLH_DNA-bd_sf InterPro; IPR032647; Mlx PANTHER; PTHR15741:SF25; PTHR15741:SF25; 1 Pfam; PF00010; HLH; 1 SMART; SM00353; HLH; 1 SUPFAM; SSF47459; SSF47459; 1 PROSITE; PS50888; BHLH; 1 1: Evidence at protein level; Activator; Alternative splicing; Complete proteome; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation CHAIN 1 298 Max-like protein X /FTId=PRO_0000127279 DOMAIN 129 187 bHLH. {ECO:0000255|PROSITE- ProRule:PRU00981} REGION 140 160 Leucine-zipper MOD_RES 7 7 Phosphoserine MOD_RES 45 45 Phosphoserine {ECO:0000250|UniProtKB:O08609} MOD_RES 48 48 Phosphoserine {ECO:0000250|UniProtKB:O08609} MOD_RES 74 74 Phosphoserine MOD_RES 77 77 Phosphoserine MOD_RES 98 98 Phosphoserine VAR_SEQ 15 68 Missing (in isoform Alpha and isoform Beta). {ECO:0000303|PubMed:10593926, ECO:0000303|PubMed:10918583, ECO:0000303|PubMed:14702039, ECO:0000303|PubMed:15489334, ECO:0000303|Ref.4} /FTId=VSP_002137 VAR_SEQ 81 110 Missing (in isoform Alpha) /FTId=VSP_002138 VARIANT 223 223 Q -> R (in dbSNP:rs665268) /FTId=VAR_049547 CONFLICT 247 247 D -> V (in Ref. 1; AAF14638) CONFLICT 298 298 Y -> C (in Ref. 3; BAA90977) SEQUENCE 298 AA; 33300 MW; D1981730051473C5 CRC64; MTEPGASPED PWVKASPVGA HAGEGRAGRA RARRGAGRRG ASLLSPKSPT LSVPRGCRED SSHPACAKVE YAYSDNSLDP GLFVESTRKG SVVSRANSIG STSASSVPNT DDEDSDYHQE AYKESYKDRR RRAHTQAEQK RRDAIKRGYD DLQTIVPTCQ QQDFSIGSQK LSKAIVLQKT IDYIQFLHKE KKKQEEEVST LRKDVTALKI MKVNYEQIVK AHQDNPHEGE DQVSDQVKFN VFQGIMDSLF QSFNASISVA SFQELSACVF SWIEEHCKPQ TLREIVIGVL HQLKNQLY
Research Areas
Citations (0)

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MLX belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This protein may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4.


Meroni,G.,, Oncogene 19 (29), 3266-3277 (2000)
Billin,A.N.,, J. Biol. Chem. 274 (51), 36344-36350 (1999)

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Cat# AP8638c
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