|Application ||WB, E|
|Calculated MW||52186 Da|
|Antigen Region||123-151 aa|
|Other Names||General transcription factor IIH subunit 4, Basic transcription factor 2 52 kDa subunit, BTF2 p52, General transcription factor IIH polypeptide 4, TFIIH basal transcription factor complex p52 subunit, GTF2H4|
|Target/Specificity||This GTF2H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-151 amino acids from the Central region of human GTF2H4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GTF2H4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre- initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.|
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Provided below are standard protocols that you may find useful for product applications.
Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
Blau,J., et.al., Mol. Cell. Biol. 16 (5), 2044-2055 (1996)
Zhou,Q. et.al., Science 274 (5287), 605-610 (1996)
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