|Application ||WB, IHC-P, FC, E|
|Other Accession||Q9QZF2, G3X745|
|Calculated MW||61680 Da|
|Antigen Region||6-31 aa|
|Other Names||Glypican-1, Secreted glypican-1, GPC1|
|Target/Specificity||This Glypican-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-31 amino acids from the N-terminal region of human Glypican-1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Glypican-1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Endosome. Note=S-nitrosylated form recycled in endosomes. Localizes to CAV1-containing vesicles close to the cell surface. Cleavage of heparan sulfate side chains takes place mainly in late endosomes. Associates with both forms of PRNP in lipid rafts. Colocalizes with APP in perinuclear compartments and with CP in intracellular compartments. Associates with fibrillar APP amyloid-beta peptides in lipid rafts in Alzheimer disease brains|
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Provided below are standard protocols that you may find useful for product applications.
GPC1(Glypican 1) is a cell surface proteoglycan that contains heparan sulphate. The protein is attached to the cell membrane by a GPI anchor. GPC1 is required for efficient TGF-beta1 signalling in pancreatic cancer cells. Members of the glypican-related integral membrane proteoglycan family (GRIPS) may play a role in the control of cell division and growth regulation.
Sjoeblom T., et.al., Science 314:268-274(2006).
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