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AFMID Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - AFMID Antibody (N-term) AP9175a
    Western blot analysis of AFMID Antibody (N-term) (Cat. #AP9175a) in mouse liver tissue lysates (35ug/lane). AFMID (arrow) was detected using the purified Pab.
  • FC - AFMID Antibody (N-term) AP9175a
    AFMID Antibody (N-term) (Cat. #AP9175a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q63HM1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33992 Da
Antigen Region 57-83 aa
Additional Information
Gene ID 125061
Other Names Kynurenine formamidase {ECO:0000255|HAMAP-Rule:MF_03014}, KFA {ECO:0000255|HAMAP-Rule:MF_03014}, KFase {ECO:0000255|HAMAP-Rule:MF_03014}, 3519 {ECO:0000255|HAMAP-Rule:MF_03014}, Arylformamidase {ECO:0000255|HAMAP-Rule:MF_03014}, N-formylkynurenine formamidase {ECO:0000255|HAMAP-Rule:MF_03014}, FKF {ECO:0000255|HAMAP-Rule:MF_03014}, AFMID {ECO:0000255|HAMAP-Rule:MF_03014}
Target/Specificity This AFMID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-83 amino acids from the N-terminal region of human AFMID.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAFMID Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AFMID {ECO:0000255|HAMAP-Rule:MF_03014}
Function Catalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.
Cellular Location Cytoplasm, cytosol {ECO:0000255|HAMAP- Rule:MF_03014}. Nucleus {ECO:0000255|HAMAP-Rule:MF_03014} Note=Predominantly cytosolic. Some fraction is nuclear {ECO:0000255|HAMAP-Rule:MF_03014}
Research Areas
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Magni,G.,, Cell. Mol. Life Sci. 61 (1), 19-34 (2004)

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Cat# AP9175a
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