|Application ||WB, FC, E|
|Calculated MW||52271 Da|
|Antigen Region||20-47 aa|
|Other Names||Acidic mammalian chitinase, AMCase, Lung-specific protein TSA1902, CHIA|
|Target/Specificity||This CHIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-47 amino acids from the N-terminal region of human CHIA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CHIA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.|
|Cellular Location||Isoform 1: Secreted. Note=Secretion depends on EGFR activity Isoform 3: Cytoplasm.|
|Tissue Location||Detected in lung epithelial cells from asthma patients (at protein level). Highly expressed in stomach. Detected at lower levels in lung.|
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Provided below are standard protocols that you may find useful for product applications.
CHIA degrades chitin and chitotriose. May participate in the defense against nematodes and other pathogens. There are 3 named isoforms produced by alternative splicing. CHIA is induced via a T helper-2 (Th2)-specific, interleukin-13-mediated pathway in epithelial cells and macrophages. CHIA may be an important mediator of IL13-induced responses in Th2-dominated disorders such as asthma. CHIA hydrolysis N-acetyl-beta-D-glucosaminide 1,4-beta-linkages in chitin and chitodextrins.
Wu, A.C., et al. J. Allergy Clin. Immunol. 125(3):754-757(2010)
Ober, C., et al. Curr Opin Allergy Clin Immunol 9(5):401-408(2009)
Seibold, M.A., et al. J. Biol. Chem. 284(29):19650-19658(2009)
Hartl, D., et al. J. Immunol. 182(8):5098-5106(2009)
Lalaker, A., et al. Am J Rhinol Allergy 23(1):8-14(2009)
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