|Application ||FC, WB, E|
|Calculated MW||38947 Da|
|Antigen Region||87-114 aa|
|Other Names||Poly(ADP-ribose) glycohydrolase ARH3, ADP-ribosylhydrolase 3, [Protein ADP-ribosylarginine] hydrolase-like protein 2, ADPRHL2, ARH3|
|Target/Specificity||This ADPRHL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-114 amino acids from the N-terminal region of human ADPRHL2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ADPRHL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ADP-ribose glycohydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine, free poly(ADP-ribose) and O- acetyl-ADP-D-ribose (PubMed:21498885, PubMed:30045870, PubMed:29907568, PubMed:30401461). Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP- ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response (PubMed:28650317, PubMed:29234005, PubMed:30045870). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:29480802). Does not hydrolyze ADP-ribosyl- arginine, -cysteine, -diphthamide, or -asparagine bonds (PubMed:16278211). Also able to degrade protein free poly(ADP- ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)- dependent cell death, a process named parthanatos (PubMed:16278211). Also hydrolyzes free poly(ADP-ribose) in mitochondria (PubMed:22433848). Specifically digests O-acetyl-ADP- D-ribose, a product of deacetylation reactions catalyzed by sirtuins (PubMed:17075046, PubMed:21498885). Specifically degrades 1''-O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D- ribose or 3''-O-acetyl-ADP-D-ribose isomers (PubMed:21498885).|
|Cellular Location||Nucleus. Cytoplasm. Chromosome. Mitochondrion matrix Note=Recruited to DNA lesion regions following DNA damage; ADP-D- ribose-recognition is required for recruitment to DNA damage sites.|
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Provided below are standard protocols that you may find useful for product applications.
ADPRHL2 is a member of the ADP-ribosylglycohydrolase family. The enzyme catalyzes the removal of ADP-ribose from ADP-ribosylated proteins. This enzyme localizes to the mitochondria, in addition to the nucleus and cytoplasm.
Niere, M., et al. Mol. Cell. Biol. 28(2):814-824(2008)
Ono, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(45):16687-16691(2006)
Mueller-Dieckmann, C., et al. Proc. Natl. Acad. Sci. U.S.A. 103(41):15026-15031(2006)
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