- CITATIONS: 1
|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8R496, Q5IBH7|
|Calculated MW||36463 Da|
|Antigen Region||147-175 aa|
|Other Names||Speedy protein A, Rapid inducer of G2/M progression in oocytes A, RINGO A, hSpy/Ringo A, Speedy-1, Spy1, SPDYA (HGNC:30613)|
|Target/Specificity||This SPDYA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the Central region of human SPDYA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPDYA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:12972555). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T- loop that obstructs the substrate-binding cleft prior to kinase activation (PubMed:28666995). Mediates cell survival during the DNA damage process through activation of CDK2 (PubMed:12839962).|
|Tissue Location||Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase|
Provided below are standard protocols that you may find useful for product applications.
SPDYA (speedy homolog A (Drosophila)) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. SPDYA can activate CDK2 without promoting CDK2 phosphorylation. SPDYA mediates cell survival during the DNA damage process through activation of CDK2.
Ke, Q., et al. Exp. Mol. Pathol. 87(3):167-172(2009)
Dinarina, A., et al. FEBS Lett. 583(17):2772-2778(2009)
McAndrew, C.W., et al. Cell Cycle 8(1):66-75(2009)
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