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SPDYA Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPDYA Antibody (Center) AP9978a
    Anti-SPDYA Antibody (Center) at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - SPDYA Antibody (Center) AP9978a
    SPDYA Antibody (Center) (Cat. #AP9978a) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPDYA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - SPDYA Antibody (Center) AP9978a
    SPDYA Antibody (Center) (Cat. #AP9978a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - SPDYA Antibody (Center) AP9978a
    Western blot analysis of SPDYA (arrow) using rabbit polyclonal SPDYA Antibody (Center) (Cat. #AP9978a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPDYA gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5MJ70
Other Accession Q8R496, Q5IBH7
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36463 Da
Antigen Region 147-175 aa
Additional Information
Gene ID 245711
Other Names Speedy protein A, Rapid inducer of G2/M progression in oocytes A, RINGO A, hSpy/Ringo A, Speedy-1, Spy1, SPDYA (HGNC:30613)
Target/Specificity This SPDYA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the Central region of human SPDYA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPDYA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPDYA (HGNC:30613)
Function Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:12972555). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T- loop that obstructs the substrate-binding cleft prior to kinase activation (PubMed:28666995). Mediates cell survival during the DNA damage process through activation of CDK2 (PubMed:12839962).
Cellular Location Nucleus
Tissue Location Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase
Research Areas
Citations ( 0 )


SPDYA (speedy homolog A (Drosophila)) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. SPDYA can activate CDK2 without promoting CDK2 phosphorylation. SPDYA mediates cell survival during the DNA damage process through activation of CDK2.


Ke, Q., et al. Exp. Mol. Pathol. 87(3):167-172(2009)
Dinarina, A., et al. FEBS Lett. 583(17):2772-2778(2009)
McAndrew, C.W., et al. Cell Cycle 8(1):66-75(2009)

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$ 325.00
$ 109.00
Cat# AP9978a
Availability: In Stock
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