|Application ||WB, IF, ICC, E|
|Other Accession||NP_997244, 79749430|
|Calculated MW||351157 Da|
|Application Notes||FREM2 antibody was raised against a 19 amino acid peptide near the center of human FREM2. Antibody can also be used for immunocytochemistry starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||FREM2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||FREM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. May be required for epidermal adhesion.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein; Extracellular side|
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Provided below are standard protocols that you may find useful for product applications.
FREM2 Antibody: FREM2 is a member of the FRAS1-related extracellular matrix protein family and like FREM1, mutations in the FREM2 gene are associated with Fraser syndrome. FREM2 localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions during morphogenetic processes and is thought to be required for maintaining the integrity of the skin epithelium and the differentiated state of renal epithelia. The FREM2 gene is one of several genes whose transcription is affecteded by TFAP2C, a transcription factor involved in mammary development, differentiation, and oncogenesis.
Jadjeda S, Smyth I, Pitera JE, et al. Identification of a new gene mutated in Fraser syndrome and mouse myelencephalic blebs. Nat. Genet.2005; 37:520-5.
Pavlakis E, Makrygiannis AK, Chiokati R, et al. Differential localization profile of Fras1/Frem proteins in epithelial basement membranes of newbord and adult mice. Histochem. Cell Biol.2008; 130:785-93.
Timmer JR, Mak TW, Manova K, et al. Tissue morphogenesis and vascular stability require the Frem2 protein, product of the mouse myelencephalic blebs gene. Proc. Natl. Acad. Sci. USA2005; 102:11746-50.
Woodfield GW, Chen Y, Bair TB, et al. Identification of primary gene targets of TFAP2C in hormone responsive breast carcinoma cells. Genes Chromosomes Cancer2010; epub.
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