|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055319, 7657496|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||79371 Da|
|Application Notes||RABEX5 antibody can be used for detection of RABEX5 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||RABGEF1; At least four isoforms of RABEX5 are known to exist; this antibody will recognize all four. RABEX5 antibody is predicted to not cross-react with other LRRFIP family members.|
|Reconstitution & Storage||RABEX5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||RABEX5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase (By similarity).|
|Cellular Location||Cytoplasm. Early endosome. Recycling endosome|
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Provided below are standard protocols that you may find useful for product applications.
RABEX5 Antibody: RABEX5 is a guanine nucleotide exchange factor (GEF) that forms a complex with rabaptin-5 and specifically activates Rab5, a small GTPase that plays an essential role in endocytic traffic. RABEX-5 is thought to also interact with another member of the Rab family, Rab22, which targets RABEX5 to early endosomes, allowing it to activate Rab5 and thereby promote Rab5-mediated endosome fusion.
Horiuchi H, Lippe R, McBride HM, et al. A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function. Cell 1997; 90:1149-59.
Zhu H, Liang Z, and Li G. Rabex-5 is a Rab22 effector and mediates a Rab22-Rab5 signaling cascade in endocytosis. Mol. Biol. Cell 2009; 20:4720-9.
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