|Application ||WB, IF, ICC, E|
|Other Accession||Q9NVI7, 283436220|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||71369 Da|
|Application Notes||ATAD3A antibody can be used for detection of ATAD3A by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||ATAD3A; ATAD3A antibody is predicted to not cross-react with other ATAD protein family members. At least two isoforms are known to exist.|
|Reconstitution & Storage||ATAD3A antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ATAD3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important role in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis.|
|Cellular Location||Mitochondrion inner membrane; Single- pass membrane protein. Mitochondrion matrix, mitochondrion nucleoid. Note=In the mitochondrial inner membrane, enriched in sites with the potential to form contacts with the outer membrane (PubMed:20349121, PubMed:20154147). The N- terminal domain interacts with the inner surface of the mitochondrial outer membrane and the C-terminal domain localizes in a specific matrix compartment, where it is associated with nucleoids (PubMed:18063578).|
|Tissue Location||Overexpressed in lung adenocarcinomas (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
ATAD3A Antibody: ATAD3A is a member of the AAA ATPase family, a family of proteins that catalyze ATP into ADP and are involved in several cellular functions such as cell-cycle regulation, protein proteolysis and transport. The AAA ATPase family is characterized by a highly conserved AAA motif containing Walker homology sequences and imparting ATPase activity. Mitochondrial membrane proteins ATAD3A/B contribute to the stabilization of nucleoids and may participate in the transformation pathway and the chemosensitivity of oligodendrogliomas. The gene encoding ATAD3A/B/C maps to human chromosome 1 and has been suggested to be an anti-apoptotic marker.
Patel S and Latterich M. The AAA team: related ATPases with diverse functions. Trends Cell Biol. 1998; 8:65-71.
Ogura T and Wilkinson AJ. AAA+ superfamily ATPases: common structure—diverse function. Genes Cells 2001; 6:575-97.
Hubstenberger A, Labourdette G, Baudier J, et al. ATAD3A and ATAD3B are distal 1p-located genes differentially expressed in human glioma cell lines and present in vitro anti-oncogenic and chemoresistant properties. Exp. Cell Res. 2008; 314:2870-83.
Fang HY, Chang CL, Hsu SH, et al. ATPase family AAA domain-containing 3A is a novel anti-apoptotic factor in lung adenocarcinoma cells. J. Cell Sci. 2010; 123:1171-80.
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