|Application ||WB, IHC-P, IF, E|
|Other Accession||Q5VX52, 341915102|
|Calculated MW||52946 Da|
|Application Notes||SPATA1 antibody can be used for detection of SPATA1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SPATA1; At least two isoforms of SPATA1 are known to exist; this antibody will detect only the longest isoforms. SPATA1 antibody is predicted to not cross-react with other SPATA family members.|
|Reconstitution & Storage||SPATA1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SPATA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
SPATA1 Antibody: SPATA1, a novel cell-surface protein, is involved in shaping the sperm head during spermatogenesis. SPATA1 is localized at chromosome 1q21. At least three isoforms of SPATA1 are known to exist. The longest is preferentially expressed in hematopoietic tissues. Its extracellular domain contains a single immunoglobulin-like domain, and its intracellular segment has two immunoreceptor tyrosine-based inhibition motifs (ITIMs). It is thought to have an important role in hematopoietic cell signaling. The mid-sized isoform contains a short intracellular part without ITIMs, while the shortest isoform lacks the transmembrane segment and represents a potential soluble protein (2, 3).
Giesecke K, Hamann H, Stock KF, et al. Evaluation of SPATA1-associated markers for stallion fertility. Anim. Genet. 2009; 40:359-65.
Xu MJ, Zhao R, Zhao ZJ. Molecular cloning and characterization of SPAP1, an inhibitory receptor. Biochem. Biophys. Res. Commun. 2001; 280:768-75
Liu SF, He S, Liu BW, et al. Cloning and characterization of testis-specific spermatogenesis associated gene homologous to human SPATA4 in rat. Biol. Pharm. Bull. 2004; 27:1867-70.
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