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SPATA4 Antibody

  • WB - SPATA4 Antibody ASC11395
    Western blot analysis of SPATA4 in mouse stomach tissue lysate with SPATA4 antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - SPATA4 Antibody ASC11395
    Immunohistochemistry of SPATA4 in mouse testis tissue with SPATA4 antibody at 2.5 µg/mL.
  • IF - SPATA4 Antibody ASC11395
    Immunofluorescence of SPATA4 in mouse testis tissue with SPATA4 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NEY3
Other Accession NP_653245, 31543825
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application Notes SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 132851
Target/Specificity SPATA4; At least two isoforms of SPATA4 are known to exist; this antibody will detect both isoforms. SPATA4 antibody is predicted to not cross-react with SPATA protein family members
Reconstitution & Storage SPATA4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsSPATA4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms TSARG2
Function May play a role in apoptosis regulation.
Cellular Location Nucleus.
Tissue Location Highly expressed in testis, the expression is observed precisely in seminiferous tubules EMBL; AY040204; AAK72463.1; -; mRNA EMBL; BC021731; AAH21731.1; -; mRNA CCDS; CCDS3826.1; - RefSeq; NP_653245.2; NM_144644.3 UniGene; Hs.481235; - ProteinModelPortal; Q8NEY3; - SMR; Q8NEY3; - BioGrid; 126337; 4 IntAct; Q8NEY3; 2 MINT; Q8NEY3; - STRING; 9606.ENSP00000280191; - iPTMnet; Q8NEY3; - PhosphoSitePlus; Q8NEY3; - BioMuta; SPATA4; - DMDM; 48474486; - PaxDb; Q8NEY3; - PeptideAtlas; Q8NEY3; - PRIDE; Q8NEY3; - ProteomicsDB; 73237; - DNASU; 132851; - Ensembl; ENST00000280191; ENSP00000280191; ENSG00000150628 GeneID; 132851; - KEGG; hsa:132851; - UCSC; uc003iuo.2; human CTD; 132851; - DisGeNET; 132851; - EuPathDB; HostDB:ENSG00000150628.6; - GeneCards; SPATA4; - HGNC; HGNC:17333; SPATA4 HPA; HPA019220; - MIM; 609879; gene neXtProt; NX_Q8NEY3; - OpenTargets; ENSG00000150628; - PharmGKB; PA38232; - eggNOG; ENOG410IJ0V; Eukaryota eggNOG; ENOG410ZE7H; LUCA GeneTree; ENSGT00910000144159; - HOGENOM; HOG000007428; - HOVERGEN; HBG054406; - InParanoid; Q8NEY3; - OMA; REIHVKQ; - OrthoDB; 1471531at2759; - PhylomeDB; Q8NEY3; - TreeFam; TF323506; - GenomeRNAi; 132851; - PRO; PR:Q8NEY3; - Proteomes; UP000005640; Chromosome 4 Bgee; ENSG00000150628; Expressed in 93 organ(s), highest expression level in testis ExpressionAtlas; Q8NEY3; baseline and differential Genevisible; Q8NEY3; HS GO; GO:0005737; C:cytoplasm; IBA:GO_Central GO; GO:0031514; C:motile cilium; IBA:GO_Central GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell GO; GO:0060285; P:cilium-dependent cell motility; IBA:GO_Central Gene3D; 1.10.418.10; -; 1 InterPro; IPR001715; CH-domain InterPro; IPR010441; CH_2 InterPro; IPR036872; CH_dom_sf Pfam; PF06294; CH_2; 1 PROSITE; PS50021; CH; 1 1: Evidence at protein level; Complete proteome; Nucleus; Polymorphism; Reference proteome CHAIN 1 305 Spermatogenesis-associated protein 4 /FTId=PRO_0000072101 DOMAIN 49 155 Calponin-homology (CH) {ECO:0000255|PROSITE-ProRule:PRU00044} VARIANT 149 149 Y -> C (in dbSNP:rs17062589) /FTId=VAR_051369 CONFLICT 70 70 N -> D (in Ref. 3; AAH21731) SEQUENCE 305 AA; 34751 MW; 7A3D1DCDC3FAC976 CRC64; MAAAGQEKGY LTQTAAALDK SPSLSPQLAA PIRGRPKKCL VYPHAPKSSR LSRSVLRWLQ GLDLSFFPRN INRDFSNGFL IAEIFCIYYP WELELSSFEN GTSLKVKLDN WAQLEKFLAR KKFKLPKELI HGTIHCKAGV PEILIEEVYT LLTHREIKSI QDDFVNFTDY SYQMRLPLVS RSTVSKSIKD NIRLSELLSN PNMLTNELKA EFLILLHMLQ RKLGRKLNPE WFDVKPTVGE VTLNHLPAQA SGRRYNLKVK RGRVVPVLPN IGSGGSSHRE IHVKQAGQHS YYSAMKPIRN MDKKP
Research Areas
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SPATA4 Antibody: The spermatogenesis associated protein 4 (SPATA4) protein, also known as TSARG1, is highly expressed in testis, and to a much lower extent in other tissues. SPATA4 contains a highly conserved DUF1042 domain, but no significant homology with any other known protein. SPATA4 is thought to play an important role in the development of cryptorchid testis and is a testis-specific apoptosis candidate oncogene.


Liu SF, Lu GX, Liu G, et al. Cloning of a full-length cDNA of human testis-specific spermatogenic cell apoptosis inhibitor TSARG2 as a candidate oncogene. Biochem. Biophys. Res. Commun. 2004; 319:32-40
Liu SF, Ai C, Ge ZQ, et al. Molecular cloning and bioinformatic analysis of SPATA4 gene. J. Biochem. Mol. Biol. 2005; 38:739-47.

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$ 335.00
Cat# ASC11395
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