|Application ||WB, IF, E|
|Other Accession||AAH10492, 206725523|
|Calculated MW||112149 Da|
|Application Notes||SIPA1 antibody can be used for detection of EPAC1 by Western blot at 1 µg/mL. Antibody can also be used for immunofluorescence starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SIPA1; At least three isoforms of SIPA1 are known to exist; this antibody will recognize all three isoforms. This antibody is specific for SIPA1 and will not recognize the other SIPA1 family of proteins.|
|Reconstitution & Storage||SIPA1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SIPA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting them to the putatively inactive GDP-bound state (PubMed:9346962). Affects cell cycle progression (By similarity).|
|Cellular Location||Nucleus. Cytoplasm, perinuclear region Endomembrane system; Peripheral membrane protein. Note=Mostly localized in the perinuclear membraneous region.|
|Tissue Location||Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord|
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Provided below are standard protocols that you may find useful for product applications.
SIPA1 Antibody: Signal-induced proliferation 1 (SIPA1) is a member of the SIPA1 family of RapGAPs, a family of mitogen-induced GTPase activating proteins. SIPA1 exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. When abnormally or prematurely expressed, SIPA1 may also hamper mitogen-induced cell cycle progression and may increase metastases in human cancers.
Hattori M, Tsukamoto N, Nur-e-Kamal MS, et al. Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression. Mol. Cell. Biol. 1995; 15:551-60.
Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci. 2009; 100:17-23.
Kurachi H, Wada Y, Tsukamoto N, et al. Human SPA-1 gene product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2. Segregate expression profiles from a rap1GAP gene product. J. Biol. Chem. 1997; 272:28081-8.
Ishida D, Kometani K, Yang H, et al. Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice. Cancer Cell 2003; 4:55-65.
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