|Application ||WB, IF, E|
|Other Accession||NP_001154, 22035548|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||92 kDa|
|Application Notes||APBA1 antibody can be used for detection of APBA1 by Western blot at 0.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||APBA1; At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other APBA proteins.|
|Reconstitution & Storage||APBA1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||APBA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the amyloid-beta precursor protein (APP) and hence formation of APP- beta.|
|Cellular Location||Cytoplasm. Cytoplasm, perinuclear region Nucleus. Note=Only about 5% of the protein is located in the nucleus|
|Tissue Location||Brain and spinal cord. Isoform 2 is expressed in testis and brain, but not detected in lung, liver or spleen|
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Provided below are standard protocols that you may find useful for product applications.
APBA1 Antibody: The Amyloid beta 4 (A4) precursor protein-binding family A member 1 (APBA1) is a member of the X11 protein family and a neuronal adapter protein that interacts with the Alzheimer's disease amyloid precursor protein (APP). APBA1 stabilizes APP and inhibits production of proteolytic APP fragments including the Aβ peptide that is deposited in the brains of Alzheimer's disease patients. It is thought to be involved in signal transduction processes and is a putative vesicular trafficking protein in the brain that can form a complex with the potential to couple synaptic vesicle exocytosis to neuronal cell adhesion. APBA1 can also bind to CASK, a multidomain scaffolding protein involved in brain development and synapse formation.
Borg JP, Ooi J, Levy E, et al. The phosphotyrosine interaction domains of X11 and FE65 bind to distinct sites on the YENPTY motif of amyloid precursor protein. Mol. Cell. Biol. 1996; 16:6229-41.
Mueller HT, Borg JP, Margolis B, et al. Modulation of amyloid precursor protein metabolism by X11alpha/Mint1. A deletion analysis of protein-protein interaction domains. J. Biol. Chem. 2000; 275:39302-6.
Maximov A, Sudhof TC, and Bezprozvanny I. Association of neuronal calcium channels with modular adaptor proteins. J. Biol. Chem. 1999; 274:24453-6
Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.
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