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CRB2 Antibody

  • WB - CRB2 Antibody ASC11590
    Western blot analysis of CRB2 in rat brain tissue lysate with CRB2 antibody at 1 µg/mL
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5IJ48
Other Accession NP_775960, 112420992
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 141 kDa
Application Notes CRB2 antibody can be used for detection of CRB2 by Western blot at 1 - 2 µg/mL.
Additional Information
Gene ID 286204
Target/Specificity CRB2; At least three isoforms of CRB2 are known to exist; this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family.
Reconstitution & Storage CRB2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsCRB2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CRB2 (HGNC:18688)
Function Apical polarity protein that plays a central role during the epithelial-to-mesenchymal transition (EMT) at gastrulation, when newly specified mesodermal cells move inside the embryo. Acts by promoting cell ingression, the process by which cells leave the epithelial epiblast and move inside the embryo to form a new tissue layer. The anisotropic distribution of CRB2 and MYH10/myosin-IIB at cell edges define which cells will ingress: cells with high apical CRB2 are probably extruded from the epiblast by neighboring cells with high levels of apical MYH10/myosin-IIB. Also required for maintenance of the apical polarity complex during development of the cortex.
Cellular Location Isoform 1: Apical cell membrane {ECO:0000250|UniProtKB:Q80YA8}; Single-pass type I membrane protein. Note=O-glucosylation is required for localization at the apical plasma membrane {ECO:0000250|UniProtKB:Q80YA8}
Tissue Location Expressed in retina, fetal eye and brain. Also expressed in kidney, ARPE-19 and RPE/choroid cell lines, and at low levels in lung, placenta, and heart
Research Areas
Citations (0)

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CRB2 Antibody: CRB2 (Crumbs homolog 2), like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.


van den Hurk J, Rashbass P, Roepman R, et al. Characterization of the Crumbs homolog 2 (CRB2) gene and analysis of its role in retinits pigmentosa and Leber congenital amaurosis. Mol. Viso. 2005; 11:263-73
Bulgakova NA and Knust E. The Crumbs complex: from epithelial-cell polarity to retinal degeneration. J. Cell Sci. 2009; 122:2587-96.
Boroviak T and Rashbass P. The apical polarity determinant Crumbs 2 is a novel regulator of ESC-derived neural progenitors. Stem Cells 2011; 29:193-205
Bujakowska K, Audo I, Mohand-Said S, et al. CRB1 mutations in inherited retinal dystrophies. Hum. Mutat. 2012; 33:306-15

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$ 335.00
Cat# ASC11590
Availability: 5-7days
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