|Application ||WB, IHC-P, IF, E|
|Other Accession||P15056, 50403720|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 84 |
Observed: 99, multiple post-translational modifications, commonly observed at 95-100kDa
|Application Notes||B-raf antibody can be used for detection of B-raf by Western blot at 1 and 2 µg/mL.|
|Target/Specificity||BRAF; At least two isoforms of B-raf are known to exist; this antibody will detect both isoforms. This antibody will not cross-react with C-raf.|
|Reconstitution & Storage||B-raf antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||B-raf Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.|
|Cellular Location||Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.|
|Tissue Location||Brain and testis.|
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Provided below are standard protocols that you may find useful for product applications.
B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
McCubrey JA, Steelman LS, Chappell WH, et al. Roles of the RAF/MEK/ERK pathway in cell growth, malignant transformation and drug resistance. Biochim. Biophys. Acta 2007; 1773:1263-84.
Madhunapantula SV and Robertson GP. Is B-raf a good therapeutic target for melanoma and other malignancies? Cancer Res. 2008; 68:5-8.
Sarkozy A, Carta C, Moretti S, et al. Germline BRAF mutations in Noonan, LEOPARD, and cardiofaciocutaneous syndromes: molecular diversity and associated phenotypic spectrum. Hum. Mutat. 2009; 30:695-702.
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